Bacteria, elemental sulfur and stable carbon isotope rations in the mangrove mud clam Anodontia Edentula (Linne, 1758) / (Record no. 9630)

MARC details
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fixed length control field 04591nam a2200301 a 4500
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control field OSt
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control field 20230921135730.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 160620s19uu xx 00 eng d
040 ## - CATALOGING SOURCE
Transcribing agency CvSU Main Campus Library
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 577
Item number L49 1999
100 #0 - MAIN ENTRY--PERSONAL NAME
Personal name Lebata, Ma. Junemie Hazel L.
Relator term author
9 (RLIN) 11884
245 10 - TITLE STATEMENT
Title Bacteria, elemental sulfur and stable carbon isotope rations in the mangrove mud clam Anodontia Edentula (Linne, 1758) /
Statement of responsibility, etc. by Ma. Junemie Hazel L. Lebata.
260 0# - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Visayas :
Date of publication, distribution, etc. 1999.
Name of publisher, distributor, etc. University of the Philippines Visayas,
300 ## - PHYSICAL DESCRIPTION
Extent xii, 64 pages :
Other physical details illustrations ;
Dimensions 28 cm.
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
500 ## - GENERAL NOTE
General note Thesis (Master of Science in Fisheries Biology) University of the Philippines in the Visayas
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes bibliographical references.
520 3# - SUMMARY, ETC.
Summary, etc. LEBATA, MA. JUNEMIE HAZEL L. University of the Philippines in the Visayas, November 1998. Bacteria, elemental sulfur and stable carbon isotope ratios in the mangrove mud clam Anodontia edentula (Linné, 1758). Thesis Adviser: Dr. Jose A. Ingles<br/><br/>Different sizes of the mangrove mud clam Anodontia edentula were collected from the mangroves in Brgy. San Roque in Estancia, Iloilo and examined for the presence of gut through dissection and microscopic observation. The presence of bacteria in the mantle, gill and foot tissues was also examined through bacterial culture and scanning electron microscopy (SEM). Elemental sulfur content of the mantle, gill and foot tissues and total sulfur content of the sediment were analyzed; and stable carbon isotope ratios of the clams, sediment, detritus and plankton in the study area were determined. These experiments were conducted to establish the possibility of clam-bacteria symbiosis in this species.<br/><br/>Dissection and histological sections of juvenile and adult mangrove clams showed the presence of a very simple gut, unlike the well-defined organs of the digestive system of other bivalve species that have no symbionts.<br/><br/>A decreasing bacterial count (CFU/g tissue) with increasing size of clam was observed only in plates containing gill isolates. In contrast, no bacterial colonies were observed in the mantle and foot isolates. The results paralleled the observations in <br/>other lucinids that bacteria are confined only in the gills.<br/><br/>Moreover, as the clam grows it becomes less dependent on the bacteria. The morphology of colonies was the same in all plates suggesting only one type of colony. Bacteria observed were gram-negative, colorless and spherical to rod shaped. SEM also revealed only one type of spherical bacteria both in cultured plates and in gill bacteriocytes.<br/><br/>Sulfur analysis showed highly significant (p<0.0001) amounts of elemental sulfur in the gills (1.38-1907.20 pmoles/g FW) compared with the quantities observed in the mantle (0.51-6.41 pmoles/g FW). Elemental sulfur was absent from the foot tissues. <br/><br/>This finding supports the aforementioned presence of bacterial symbionts only in the gills. Furthermore, results showed a significantly (p<0.0001) decreasing elemental sulfur from the newly collected clams compared to those maintained in mangrove mud and stocked in seawater only which were analyzed 3 weeks later indicating that stored elemental sulfur is being utilized by the bacteria in the absence of sulfide.<br/><br/>Moreover, smaller clams (40.1-50.0 mm SL) had significantly higher elemental sulfur content than bigger ones (50.1-60.0 mm SL) supporting the inverse relationship between number of gill bacteria and clam size. Total sulfur content of mangrove mud in situ was higher than those used as substrate in the experiment; and was not significantly different from initial to final readings in the latter. This shows that mangrove mud in situ is linked to a steady sulfur source.<br/><br/>Stable C isotope ratios showed that the clams do not obtain their carbon from plankton, sediment nor detritus and may thus be solely dependent on bacterial carbon. This was shown by the more negative 613C ratios of carbon fixed during bacterial chemoautotrophy compared to carbon fixed photosynthetically.<br/><br/><br/>
541 ## - IMMEDIATE SOURCE OF ACQUISITION NOTE
Method of acquisition Submitted to the University Library
Date of acquisition 08/04/2020
Accession number T-5947
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Ecology
9 (RLIN) 1442
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
9 (RLIN) 11885
Course or Program Master of Science in Fisheries Biology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Ingles, Jose A.
9 (RLIN) 38550
Relator term adviser
856 ## - ELECTRONIC LOCATION AND ACCESS
Electronic File <a href="http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=0738e52da3f27d3828589347013b6c0e">http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=0738e52da3f27d3828589347013b6c0e</a>
Link text Click here to view thesis abstract and table of contents
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Theses / Manuscripts
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Home library Current library Shelving location Date acquired Source of acquisition Coded location qualifier Inventory number Total Renewals Full call number Barcode Date last seen Uniform Resource Identifier Price effective from Koha item type Public note
    Dewey Decimal Classification   Room use only Non-fiction Ladislao N. Diwa Memorial Library Ladislao N. Diwa Memorial Library Theses Section 08/04/2020 Submitted copy to the University Library THESIS/SP 500 1999 577 L49 1999 00009628 08/04/2020 http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=0738e52da3f27d3828589347013b6c0e 08/04/2020 Theses / Manuscripts T-5947
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