In vitro acaricidal activity of talahib (Saccharum spontaneum) stem extract against adult brown dog ticks (Rhipicephalus sanguineus) / by Janielle M. Fajardo and Adriana C. Garcia.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2016. Cavite State University- Main Campus,Description: xiii, 51 pages : 28 cm. illustrations ; Content type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 615  F17 2016
Online resources: Production credits:
  • College of Nursing (CON)
Abstract: FAJARDO JANIELLE M., GARCIA ADRIANA C., In vitro Acaricidal Activity of Talahib (Saccharum spontaneum) Stem Extract Against Adult Brown Dog Ticks (Rhipicephalus saguineus). Undergraduate Thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang Cavite. Adviser: Dr. Adelida E. Sangalang. The study was conducted from May to June 2016 at the laboratory of the Department of Medical Technology, College of Nursing, Cavite State University, Bancod, Indang, Cavite to determine the in vitro acaricidal activity of talahib (Saccharum spontaneum) stem extract against adult brown dog ticks (Rhipicephalus saguineus). Specific objectives of the study aimed to: (1) determine the mortality rate of R. sanguineus treated with prepared S. spontaneum, aqueous and ethanolic stem extracts with different concentration at different time intervals; (2) determine what concentration of the prepared extracts is most effective in killing ticks; (3) determine if the prepared S. spontaneumextracts is comparable with the commercially available acaricides; (4) determine the toxicity of the prepared extracts. A Completely Randomized Design was used allocating the ticks to eight treatments namely Treatment 1- Frontline (positive control), Treatment 2- Distilled water (negative control), Treatment 3- S. spontaneum 30% aqueous extract, Treatment 4- S. spontaneum 40% aqueous extract, Treatment 5- S. spontaneum 50% aqueous extract, Treatment 6- S. spon/aneum 30% ethanolic extract, Treatment 7- S. spontaneum 40% ethanolic extract, Treatment 8- S. spontaneum 50% ethanolic extract. Each treatment was replicated three times with number of thirty ticks per replicate. Observation was made and numbers of dead ticks were counted on each given post exposure time. Findings of the study revealed that the effectiveness of the talahib extracts, and the mortality of the ticks varied according to the solvent, its concentration and the time of exposure of the ticks to the treatments. The study showed that the 40 and 50 percent ethanolic extracts are the most effective treatments having no significant difference. The toxicity level was assessed using probit analysis of the lethal concentration 50, (LC 50) and lethal concentration 90 (LC 90). The lowest concentration which was the S. spontaneum stem extract with 30 percent ethanolic solution was subjected to probit analysis; result showed that the stem extract was higher compared to other studies. Based on the results gathered the general objective of the study showed that in vitro acaricidal activity of talahib (Saccharum spontaneum) stem extract against adult brown dog tick (Rhipicephalus saguineus) was effective. However, the researchers were highly recommending to use lower concentrations so it could be subjected to in vivo analysis.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 615 F17 2016 (Browse shelf(Opens below)) Link to resource Room use only T-6609 00010886

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

FAJARDO JANIELLE M., GARCIA ADRIANA C., In vitro Acaricidal Activity of Talahib (Saccharum spontaneum) Stem Extract Against Adult Brown Dog Ticks (Rhipicephalus saguineus). Undergraduate Thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang Cavite. Adviser: Dr. Adelida E. Sangalang.
The study was conducted from May to June 2016 at the laboratory of the Department of Medical Technology, College of Nursing, Cavite State University, Bancod, Indang, Cavite to determine the in vitro acaricidal activity of talahib (Saccharum spontaneum) stem extract against adult brown dog ticks (Rhipicephalus saguineus). Specific objectives of the study aimed to: (1) determine the mortality rate of R.
sanguineus treated with prepared S. spontaneum, aqueous and ethanolic stem extracts with different concentration at different time intervals; (2) determine what concentration of the prepared extracts is most effective in killing ticks; (3) determine if the prepared S.
spontaneumextracts is comparable with the commercially available acaricides; (4) determine the toxicity of the prepared extracts.
A Completely Randomized Design was used allocating the ticks to eight treatments namely Treatment 1- Frontline (positive control), Treatment 2- Distilled water (negative control), Treatment 3- S. spontaneum 30% aqueous extract, Treatment 4- S.
spontaneum 40% aqueous extract, Treatment 5- S. spontaneum 50% aqueous extract, Treatment 6- S. spon/aneum 30% ethanolic extract, Treatment 7- S. spontaneum 40% ethanolic extract, Treatment 8- S. spontaneum 50% ethanolic extract. Each treatment was replicated three times with number of thirty ticks per replicate. Observation was made and numbers of dead ticks were counted on each given post exposure time.
Findings of the study revealed that the effectiveness of the talahib extracts, and the mortality of the ticks varied according to the solvent, its concentration and the time of exposure of the ticks to the treatments. The study showed that the 40 and 50 percent ethanolic extracts are the most effective treatments having no significant difference. The toxicity level was assessed using probit analysis of the lethal concentration 50, (LC 50) and lethal concentration 90 (LC 90). The lowest concentration which was the S.
spontaneum stem extract with 30 percent ethanolic solution was subjected to probit analysis; result showed that the stem extract was higher compared to other studies.
Based on the results gathered the general objective of the study showed that in vitro acaricidal activity of talahib (Saccharum spontaneum) stem extract against adult brown dog tick (Rhipicephalus saguineus) was effective. However, the researchers were highly recommending to use lower concentrations so it could be subjected to in vivo analysis.

Submitted copy to the University Library. 05/08/2017 T-6609

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