Effect of grapefruit (Citrus paradisi) peel and rind extracts against Ascaris suis / by Arianelyn B. Esplanada, Jen-Marianne F. Gloriani and Sivel Marie J.Tinte.Material type: TextLanguage: English Publication details: Indang, Cavite : 2016. Cavite State University- Main Campus,Description: xv, 56 pages : illustrations ; 28 cmContent type:
- 577.8 Es6 2016
- College of Nursing (CON)
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Thesis (BS Medical Technology) Cavite State University
Includes bibliographical references.
College of Nursing (CON)
ESPLANADA, ARIANE LYN B., GLORIANI, JEN-MARIANNE F., TINTE,
SIVEL MARIE J. Effects of Grapefruit (Citrus paradisi) Peel and Rind Extract Against Ascaris suis. Undergraduate Thesis. Bachelor of Science in Medical Technology. College of Nursing, Cavite State University, Indang, Cavite. November 2016. Adviser: Chester Joshua V. Saldana DVM, MSc and Flordeliza De Guzman, RMT, MAT.
A study was conducted to determine the effects of Grapefruit (Citrus paradisi) against Ascaris suis. It also aimed to determine the phytochemicals present in the peel
and rind extract of Grapefruit (Citrus paradisi); screen the anthelminthic effect of
extracts in vitro; and determine the concentration of extract that will exhibit 50 percent and 100 percent mortality on the test organism.
One hundred eleven pieces of Grapefruit (Citrus paradisi) were used and extracted by cold pressing. The extracts were subjected to phytochemical screening. The pure (100%) Citrus paradisi peel and rind extracts were used and the remaining extracts were diluted at 25, 50 and 75 percent concentration. Anthelminthic assay was performed, the solutions and positive control and negative controls were tested parallel to the
treatment groups. Observation was done every 6 to 30 hours. Each treatment was replicated three times. Mortality of the worms at different time intervals was noted analyzed.
Results of the phytochemical screening revealed that Citrus paradisi peel and rind possess sterols, triterpenes, alkaloids, saponins, glycosides, flavonoids, and tannins. With regards to the anthelminthic activity trials, significantly lower mortality of Ascaris suis were recorded by 25 percent Citrus paradisi peel and rind extract concentration at
exposure time of 6 hours as compared with 50, 75 and 100 percent concentration. However, when the worms were exposed longer for at least 30 hours, 25 percent peel and rind extract had similar killing effect on Ascaris suis as with higher concentrations. The mortality rates of Ascaris suis suggested a clear relationship between the concentration and the exposure time. This meant the higher the concentration, the faster it killed all Ascaris suis, the longer the time of exposure, the lower the concentration needed to kill all the worms. Thus, the least Citrus paradisi peel and rind concentration that could kill all the worms the fastest at 24 hours was 75 percent, while the least peel and rind extract concentration that could kill all the worms at 30 hours or longer is 25 percent. One hundred percent mortality was recorded in all the concentrations at 30 hours post exposure. Lowest mortality rate was observed in distilled water treated Ascaris suis while application of albendazole resulted in higher mortality rate at fastest time compared to distilled water and treatment groups.
This study revealed that Citrus paradisi peel and rind extract possessed anthelmintic activity against Ascaris suis.
Submitted copy to the University Library. 05/10/2017 T-6622