Pediculicidal activity of herbal shampoo formulated from Plectranthus amboinicus and Cananga odorata extracts against Pediculus humanus capitis / by Hanna Grace D. Bersamina and Christian Jay C. Dela Rea.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2016. Cavite State University- Main Campus,Description: xviii, 110 pages : 28 cm. illustrations ; Content type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 615  B46 2016
Online resources: Production credits:
  • College of Nursing (CON)
Abstract: BERSAMINA, HANNA GRACE D., DELA REA, CHRISTIAN JAY C. Pediculicidal Activity of Herbal Shampoo Formulated from Plectranthus amboinicus and Cananga odorata Extracts Against Pediculus humanus capitis. Undergraduate Thesis. Department of Medical Technology, College of Nursing, Cavite State University, Indang, Cavite. November 2016. Adviser: Ms. Karen Krista M. Escobar, RMT, MSMT The study was conducted from March to June 2016 in the laboratory of the Department of Medical Technology at Cavite State University Indang, Cavite. This study was conducted to determine the pediculicidal activity of herbal shampoo formulated from Plectranthus amboinicus and Cananga odorata extracts against Pediculus humanus capitis. Specifically, it aimed to determine if the properties of the herbal shampoos produced are comparable to Oilganics shampoo in terms of viscosity, color, odor, foam volume and pH level; determine if the shampoo formulated from Cananga odorata (ylang ylang) extract only, Plectranthus amboinicus (oregano) extract only and combination of both plant extract will exhibit activity against Pediculus humanus capitis; determine the mortality rate of Pediculus humanus capitis per time interval in each treatment; determine the most effective treatment against Pediculus humanus capitis; and determine if the most effective treatment will cause any dermal irritation. Five kilograms of Plectranthus amboinicus leaves and five kilograms of Cananga odorata flower were collected and extracted at De La Salle University Dasmarifias Interdisciplinary Science and Research Unit in Dasmarifias, Cavite. It was then screened for presence of terpene using Salkowski test. The formulation of shampoo was done at College of Nursing, Department of Medical Technology Laboratory in Cavite State University — Main Campus, Indang, Cavite. Evaluation of shampoo and collection of Pediculus humanus capitis was done in two elementary schools in Naic, Cavite. The determination of the pediculicidal activity of the herbal shampoos was done in the same laboratory of the Medical Technology Department. Determination of the dermal irritation of the most effective treatment was done at Department of Science and Technology, Industrial Technology Development Institute, Testing Department. Eleven treatments were used in the study. The first two treatments were the control group and the other nine treatments were the experimental group. Results showed that based on viscosity, treatment 6 and treatnent 7 were the most comparable treatments to Oilganics shampoo. Based on color, only the negative control were comparable to Oilganics shampoo but none of the experimental treatment. Based on odor, treatment 6, treatment 7, treatment 8 and treatment 9 were comparable to Oilganics shampoo. For pH, treatment 7 had the closest pH. Based on foam volume, treatment 3 and treatment 6 even exceeded the foam volume of Oilganics shampoo. All treatments exhibited a pediculicidal activity against Pediculus humanus capitis with treatment 7 (50% concentration of Cananga odorata) having the highest mortality rate of 100 percent and having the fastest pediculicidal activity within fifteen minutes. Treatment 7 was the most effective however it exhibited dermal irritation according to the result released by at Department of Science and Technology, Industrial Technology Development Institute, Testing Department. The researchers would like to recommend further study of Treatment 7 as a pediculicidal shampoo, to determine the concentration of Cananga odorata that would not have a dermal irritation but could exhibit a high pediculicidal activity. Another recommendation was to formulate shampoo based on Cananga odorata with a different formulation than the one used in this study. Further recommend the study on the different parts of the Cananga odorata tree that might also exhibit pediculicidal activity and to make use of other methods of extracting Cananga odorata flower and Plecthrantus amboinicus leaf. The use of other solvents aside from methylene chloride in extracting terpene was also recommended.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 615 B46 2016 (Browse shelf(Opens below)) Link to resource Room use only T-6605 00010903

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

BERSAMINA, HANNA GRACE D., DELA REA, CHRISTIAN JAY C. Pediculicidal Activity of Herbal Shampoo Formulated from Plectranthus amboinicus and Cananga odorata Extracts Against Pediculus humanus capitis. Undergraduate Thesis. Department of Medical Technology, College of Nursing, Cavite State University, Indang, Cavite. November 2016. Adviser: Ms. Karen Krista M. Escobar, RMT, MSMT
The study was conducted from March to June 2016 in the laboratory of the Department of Medical Technology at Cavite State University Indang, Cavite. This study was conducted to determine the pediculicidal activity of herbal shampoo formulated from Plectranthus amboinicus and Cananga odorata extracts against Pediculus humanus capitis. Specifically, it aimed to determine if the properties of the herbal shampoos produced are comparable to Oilganics shampoo in terms of viscosity, color, odor, foam volume and pH level; determine if the shampoo formulated from Cananga odorata (ylang ylang) extract only, Plectranthus amboinicus (oregano) extract only and combination of both plant extract will exhibit activity against Pediculus humanus capitis; determine the mortality rate of Pediculus humanus capitis per time interval in each treatment; determine the most effective treatment against Pediculus humanus capitis; and determine if the most effective treatment will cause any dermal irritation.
Five kilograms of Plectranthus amboinicus leaves and five kilograms of Cananga odorata flower were collected and extracted at De La Salle University Dasmarifias Interdisciplinary Science and Research Unit in Dasmarifias, Cavite. It was then screened for presence of terpene using Salkowski test. The formulation of shampoo was done at
College of Nursing, Department of Medical Technology Laboratory in Cavite State
University — Main Campus, Indang, Cavite. Evaluation of shampoo and collection of
Pediculus humanus capitis was done in two elementary schools in Naic, Cavite. The
determination of the pediculicidal activity of the herbal shampoos was done in the same laboratory of the Medical Technology Department. Determination of the dermal irritation of the most effective treatment was done at Department of Science and Technology, Industrial Technology Development Institute, Testing Department. Eleven treatments were used in the study. The first two treatments were the control group and the other nine treatments were the experimental group.
Results showed that based on viscosity, treatment 6 and treatnent 7 were the most comparable treatments to Oilganics shampoo. Based on color, only the negative control were comparable to Oilganics shampoo but none of the experimental treatment. Based on odor, treatment 6, treatment 7, treatment 8 and treatment 9 were comparable to Oilganics shampoo. For pH, treatment 7 had the closest pH. Based on foam volume, treatment 3 and treatment 6 even exceeded the foam volume of Oilganics shampoo. All treatments exhibited a pediculicidal activity against Pediculus humanus capitis with treatment 7 (50% concentration of Cananga odorata) having the highest mortality rate of 100 percent and having the fastest pediculicidal activity within fifteen minutes. Treatment 7 was the most effective however it exhibited dermal irritation according to the result released by at Department of Science and Technology, Industrial Technology Development Institute, Testing Department.
The researchers would like to recommend further study of Treatment 7 as a pediculicidal shampoo, to determine the concentration of Cananga odorata that would not have a dermal irritation but could exhibit a high pediculicidal activity. Another recommendation was to formulate shampoo based on Cananga odorata with a different formulation than the one used in this study. Further recommend the study on the different parts of the Cananga odorata tree that might also exhibit pediculicidal activity and to make use of other methods of extracting Cananga odorata flower and Plecthrantus amboinicus leaf. The use of other solvents aside from methylene chloride in extracting terpene was also recommended.

Submitted copy to the University Library. 05/03/2017 T-6605

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