Synergistic antimicrobial effect of Madre de cacao (Gliricidia sepium) and guava (Psidium guajava) against Pseudomonas aeruginosa and Staphylococcus aureus / by Peter Paul Layague, Mikee F. Merlan and Gem Cyrell M. Peñalba.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2016. Cavite State University- Main Campus,Description: xiii, 82 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 615  L45 2016
Online resources: Production credits:
  • College of Nursing (CON)
Abstract: LAYAGUE, PETER PAUL V., MERLAN, MIKEL F., AND PENALBA, GEM CYRELL M. Synergistic Antimicrobial Effect of Madre de cacao (Gliricidia sepium) and Guava (Psidium guajava) against Pseudomonas aeruginosa And Staphylococcus aureus. Undergrduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, December 2016. Adviser: Ms. Isolde Quitan, RMT. The study, entitled "Synergistic Antimicrobial Effect of Madre de cacao (Gliricidia sepium) and Guava (Psidium guajava)against Pseudomonas aeruginosa and Staphylococcus aureus", was conducted at the Center for Basic Biomedical Research located at Angelo King Medical Research Building of the De La Salle Health Science Institute in Dasmarifias, Cavite from April 2016 to May 2016. The study generally intended to determine the combined effect of Madre de cacao and Guava leaves extract against Pseudomonas aeruginosa and Staphylococcus aureus. Specifically, the study aimed to: ascertain the phytochemical constituents of Madre de cacao (Gliricidia sepium) and Guava (Psidium guajava); determine its separate antimicrobial effects on P. aeruginosa and S. aureus; determine the size of colony inhibition of P. aeruginosa and S. aureus as exhibited by the combined extracts of Madre de cacao (G. sepium) and Guava (P. gucyava); and ascertain its synergistic effects of different concentrations against P. aeruginosa and S. aureus. The determination of the synergistic antimicrobial effects of Madre de cacao and Guava leaves extract against Pseudomonas aeruginosa and Staphylococcus aureus was done through treatments with separate and combined concentrations of the ethanolic leaves extract. The zone of inhibitions exhibited by Madre de cacao and Guava leaves extract were measured and interpreted the results through analysis of variance using Duncan Multiple Range Test (DMRT). The results of the study revealed that Madre de cacao leaves extractalone showed no inhibition on both S. aureus and P.aeruginosa. In contrast, the guava leaves extract was proven effective to inhibit both S. aureus and P.aeruginosa on its own. The combined extracts still showed inhibition against both organisms that was comparable to the zone of inhibitions of guava extract alone. It was also revealed that different compositions of leaves specifically Madre de cacao collected from different locale was considered as factor of its ineffectiveness against the organisms.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 615 L45 2016 (Browse shelf(Opens below)) Link to resource Room use only T-6619 00010904

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

LAYAGUE, PETER PAUL V., MERLAN, MIKEL F., AND PENALBA, GEM CYRELL M. Synergistic Antimicrobial Effect of Madre de cacao (Gliricidia sepium) and Guava (Psidium guajava) against Pseudomonas aeruginosa And Staphylococcus aureus. Undergrduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, December 2016. Adviser: Ms. Isolde Quitan, RMT.
The study, entitled "Synergistic Antimicrobial Effect of Madre de cacao (Gliricidia sepium) and Guava (Psidium guajava)against Pseudomonas aeruginosa and Staphylococcus aureus", was conducted at the Center for Basic Biomedical Research located at Angelo King Medical Research Building of the De La Salle Health Science Institute in Dasmarifias, Cavite from April 2016 to May 2016. The study generally intended to determine the combined effect of Madre de cacao and Guava leaves extract against Pseudomonas aeruginosa and Staphylococcus aureus. Specifically, the study aimed to: ascertain the phytochemical constituents of Madre de cacao (Gliricidia sepium) and Guava (Psidium guajava); determine its separate antimicrobial effects on P.
aeruginosa and S. aureus; determine the size of colony inhibition of P. aeruginosa and S. aureus as exhibited by the combined extracts of Madre de cacao (G. sepium) and Guava (P. gucyava); and ascertain its synergistic effects of different concentrations against P. aeruginosa and S. aureus.
The determination of the synergistic antimicrobial effects of Madre de cacao and Guava leaves extract against Pseudomonas aeruginosa and Staphylococcus aureus was done through treatments with separate and combined concentrations of the ethanolic leaves extract. The zone of inhibitions exhibited by Madre de cacao and Guava leaves extract were measured and interpreted the results through analysis of variance using Duncan Multiple Range Test (DMRT).
The results of the study revealed that Madre de cacao leaves extractalone showed no inhibition on both S. aureus and P.aeruginosa. In contrast, the guava leaves extract was proven effective to inhibit both S. aureus and P.aeruginosa on its own. The combined extracts still showed inhibition against both organisms that was comparable to the zone of inhibitions of guava extract alone.
It was also revealed that different compositions of leaves specifically Madre de cacao collected from different locale was considered as factor of its ineffectiveness against the organisms.

Submitted copy to the University Library. 05/10/2017 T-6619

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