Kuhol as protein source for bacterial culture / by Olivette S. Olaveria and Roselle C. Sapanghila.

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

OLAVERIA, OLIVETTE S. and SAPANGHILA, ROSELLE C. Kuhol as Protein
Source for Bacterial Culture. Undergraduate Thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite, October 2016. Adviser: Ms. Karen Krista M. Escobar.
The study entitled "Kuhol as Protein Source for Bacterial Culture" was conducted at the College of Nursing, Cavite State University, Indang, Cavite from July to August 2016. It aimed to determine the efficacy of golden apple snail (GAS) as protein source for the growth of selected gram positive bacteria and gram negative. Specifically, the purpose of this study were: (l) to identify the species of gram positive and gram negative bacteria that successfully grow on the formulated culture media; (2) to determine which among the eggs or meat of the golden apple snail has the highest bacterial colony count of gram positive and gram negative bacteria in plated culture media; (3) to determine the stage of golden apple snail's life cycle which had the most effective protein source for growth of selected gram positive and gram negative bacteria in plated culture media; (4) to determine the amount of pulverized golden apple snail eggs or meat affected the growth of selected gram positive and gram negative bacteria; and (5) to determine the difference in morphology and growth curve of the test organisms.
The study used kuhol eggs and kuhol meat as substitute for peptone, yeast and beef extract (protein source) in commercially prepared nutrient agar and nutrient broth. After the preparation of the culture media which was a broth and an agar it was inoculated with the test organisms. It was incubated for 24 hours and the growth curve for the broth was measured in terms of optical density from 30 to 120 minutes. In obtaining the bacterial colony count in each treatment the inoculated organisms in the medium was diluted into four-folds. One milliliter of tube diluted sample was plated in nutrient agar and incubated for 24 hours. Colony forming units were counted using a colony counter.
Based on the results obtained from this study, the researchers found out that in Enterobacter aerogenes, the highest colony count was observed in treatment I (commercially prepared nutrient agar). Serratia marcescens attained the highest colony count on treatment eight (with 150% kuhol meat). There was no significant difference between treatment I and treatment 8. Pseudomonas fluorescens showed the highest colony count on treatment seven (with 100% kuhol meat). Treatment 7 was significantly different than the commercially prepared nutrient agar. Staphylococcus epidermidis highest colony count was noticed on treatment eight. There was no significant difference between the commercially prepared nutrient agar and treatment eight which was the 150 percent kuhol meat. Bacillus subtilis and Bacillus coagulans presented the highest colony count on treatment eight but there was no difference in using commercially prepared nutrient agar and the varying concentrations of kuhol meat.
The growth curve of each bacterium in every treatment was nearly the same; the only difference was that in the optical density which meant that the varying concentration of kuhol meat and eggs affected its growth. Same colony morphology was also observed between commercially prepared nutrient agar and the formulated culture media.
The study recommends that kuhol meat can be a good alternative protein source for bacterial culture.

Submitted copy to the University Library. 05/10/2017 T-6618