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Antifungal spectrum of fungal endophytes isolated from young and matured leaves of coffea spp. in selected Towns of Cavite / by Kyria H. Saputil.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite: Cavite State University- Main Campus, 2019.Description: xiii, 57 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 581.23  Sa7 2019
Online resources: Production credits:
  • College of Arts and Sciences (CAS), Department of Biological Sciences.
Abstract: SAPUTIL, KYRIA H. Antifungal Spectrum of Fungal Endophytes Isolated from Young and Matured Leaves of Coffea spp. in Selected Towns of Cavite. Undergraduate Thesis. Bachelor of Science in Biology, major in Microbiology. Department of Biological Sciences, College of Arts and Sciences, Cavite State University, Indang, Cavite. June 2019. Adviser: Ms. Liwayway P. Taglinao This study was conducted to isolate and identify fungal endophytes found in young and matured healthy leaves of Coffea spp. positive for inhibition against yeast and molds. Tissue planting method was used to isolate endophytic fungi using Potato Dextrose Agar (PDA) medium amended with chloramphenicol and incubated for 7-14 days. A total of 40 fungal endophytes were isolated from young and matured leaves of Coffea spp. from coffee growing towns of Cavite, namely: Mendez, Indang, Amadeo, Silang and Alfonso. Out of these isolate fungal endophytes, 13 were obtained from the young leaves while 27 were obtained from the matured leaves. These fungal endophytes were screened for their antifungal activity against yeasts such as, Saccharomyces cerevisiae, Candida albicans and Kloeckera, and molds, Aspergillus brasiliensis, Penicillum sp., Fusarium oxysporum and Trichophyton mentagrophyte using agar plug method. One isolate from midrib of matured leaves was positive for inhibition against the A. brasiliensis and T. mentagrophytes. Fungal endophyte that has antifungal activity was identified into the genus, Geotrichum. sp.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 581.23 Sa7 2019 (Browse shelf(Opens below)) Link to resource Room use only T-8072 00077385

Thesis (Bachelor of Science in Biology Major in Microbiology) Cavite State University.

Includes bibliographical references.

College of Arts and Sciences (CAS), Department of Biological Sciences.

SAPUTIL, KYRIA H. Antifungal Spectrum of Fungal Endophytes Isolated from Young and Matured Leaves of Coffea spp. in Selected Towns of Cavite. Undergraduate Thesis. Bachelor of Science in Biology, major in Microbiology. Department of Biological Sciences, College of Arts and Sciences, Cavite State University, Indang, Cavite. June 2019. Adviser: Ms. Liwayway P. Taglinao
This study was conducted to isolate and identify fungal endophytes found in young and matured healthy leaves of Coffea spp. positive for inhibition against yeast and molds. Tissue planting method was used to isolate endophytic fungi using Potato Dextrose Agar (PDA) medium amended with chloramphenicol and incubated for 7-14 days. A total of 40 fungal endophytes were isolated from young and matured leaves of Coffea spp. from coffee growing towns of Cavite, namely: Mendez, Indang, Amadeo, Silang and Alfonso. Out of these isolate fungal endophytes, 13 were obtained from the young leaves while 27 were obtained from the matured leaves. These fungal endophytes were screened for their antifungal activity against yeasts such as, Saccharomyces cerevisiae, Candida albicans and Kloeckera, and molds, Aspergillus brasiliensis, Penicillum sp., Fusarium oxysporum and Trichophyton mentagrophyte using agar plug method. One isolate from midrib of matured leaves was positive for inhibition against the A. brasiliensis and T. mentagrophytes. Fungal endophyte that has antifungal activity was identified into the genus, Geotrichum. sp.

Submitted to the University Library July 03, 2019 T-8072

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