Occurence of keratinophilic fungi from different agricultural soils / by Jona Rose A. Lopez.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2019.Description: xiii, 83 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 589.2  L88 2019
Online resources: Production credits:
  • College of Arts and Sciences (CAS), Department of Biological Sciences.
Abstract: LOPEZ, JONA ROSE A. Occurrenceof Keratinophilic Fungifrom Different Agricultural Soils. Undergraduate Thesis. Bachelor of Science in Biology, major in Microbiology. Cavite State University, Indang, Cavite. May 2019. Adviser: Prof. Sherine M. Cruzate. Keratinophilic fungi are considered as one of the ecologically important groups of fungi because of their capability to degrade keratin. However, these microorganisms are also responsible for cutaneous skin infections known as dermatophytosis, which accounts for 25% of global skin mycoses worldwide, hence, this study. The study aimed to determine the physico-chemical properties of soils collected from the selected areas of Cavite. Second is to isolate keratinophilic fungi then identify them based on cultural, morphological, and physiological characteristics. Lastly, is to correlate the physico-chemical properties of the soil to the occurrence of the fungi. Soil samples were collected from selected farms of Amadeo, Indang, and Tagaytay City, Cavite. Fungal isolation was done through serial dilution technique and spread plate method on mineral salt medium (MSM) baited with sterilized horse hair. Occurrence of fungi was then correlated to the physico-chemical properties of the soil samples such as temperature, pH, and moisture content. The highest temperature was recorded from Barangay Mataas na Lupa, Indang, and Barangay Halang, Amadeo (23.67 °C) and the lowest was from Barangay Tolentino-East, Tagaytay City (21.5°C). As for the pH, the highest was from Barangay Mataas na Lupa (7.03), and the lowest was from Barangay Tolentino-East (5.25). For moisture content, Barangay Mataas na Lupa had the highest record (3.2%) while the lowest was from Cavite State University farm (1.5%). Forty three isolates were non-dermatophytic that belonged under the following genera: Aspergillus; Penicillium; Cladosporium; Acremonium; Beauveria; Paecilomyces; Fusarium; Phoma;and Absidia. Only one isolate was unidentified. Scatter plots showed an inverse relationship between the physico-chemical properties of the soil to the occurrence of keratinophilic fungi. However, statistical analysis showed that there was no correlation between them. This suggests that the occurrence of the keratinophilic fungi was also influenced by other conditions, which might be limiting to other physico-chemical properties of the soil, such as the nutrients needed by these microorganisms to grow.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 589.2 L88 2019 (Browse shelf(Opens below)) Link to resource Room use only T-8069 00077386

Thesis (Bachelor of Science in Biology Major in Microbiology) Cavite State University.

Includes bibliographical references.

College of Arts and Sciences (CAS), Department of Biological Sciences.

LOPEZ, JONA ROSE A. Occurrenceof Keratinophilic Fungifrom Different Agricultural Soils. Undergraduate Thesis. Bachelor of Science in Biology, major in Microbiology. Cavite State University, Indang, Cavite. May 2019. Adviser: Prof. Sherine M. Cruzate.
Keratinophilic fungi are considered as one of the ecologically important groups of fungi because of their capability to degrade keratin. However, these microorganisms are also responsible for cutaneous skin infections known as dermatophytosis, which accounts for 25% of global skin mycoses worldwide, hence, this study. The study aimed to determine the physico-chemical properties of soils collected from the selected areas of Cavite. Second is to isolate keratinophilic fungi then identify them based on cultural, morphological, and physiological characteristics. Lastly, is to correlate the physico-chemical properties of the soil to the occurrence of the fungi. Soil samples were collected from selected farms of Amadeo, Indang, and Tagaytay City, Cavite. Fungal isolation was done through serial dilution technique and spread plate method on mineral salt medium (MSM) baited with sterilized horse hair. Occurrence of fungi was then correlated to the physico-chemical properties of the soil samples such as temperature, pH, and moisture content. The highest temperature was recorded from Barangay Mataas na Lupa, Indang, and Barangay Halang, Amadeo (23.67 °C) and the lowest was from Barangay Tolentino-East, Tagaytay City (21.5°C). As for the pH, the highest was from Barangay Mataas na Lupa (7.03), and the lowest was from Barangay Tolentino-East (5.25). For moisture content, Barangay Mataas na Lupa had the highest record (3.2%) while the lowest was from Cavite State University farm (1.5%). Forty three isolates were non-dermatophytic that belonged under the following genera: Aspergillus; Penicillium; Cladosporium; Acremonium; Beauveria; Paecilomyces; Fusarium; Phoma;and Absidia. Only one isolate was unidentified. Scatter plots showed an inverse relationship between the physico-chemical properties of the soil to the occurrence of keratinophilic fungi. However, statistical analysis showed that there was no correlation between them. This suggests that the occurrence of the keratinophilic fungi was also influenced by other conditions, which might be limiting to other physico-chemical properties of the soil, such as the nutrients needed by these microorganisms to grow.

Submitted to the University Library July 03, 2019 T-8069

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