Screening and characterization of laccase-producing fungi isolated from Industrial soil samples of Cavite / by Claire M. Nati.Material type: TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2019.Description: xiv, 64 pages : illustrations ; 28 cmContent type:
- 581.23 N19 2019
- College of Arts and Sciences (CAS), Department of Biological Sciences.
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Thesis (Bachelor of Science in Biology Major in Microbiology) Cavite State University.
Includes bibliographical references.
College of Arts and Sciences (CAS), Department of Biological Sciences.
NATI, CLAIRE M. Screening and Characterization of Laccase Producing Fungi Isolated from Industrial Soil Samples of Cavite. Undergraduate Thesis. Bachelor of Science in Biology, major in Microbiology. College of Arts and Sciences. Cavite State University, Indang, Cavite. June 2019. Adviser: Prof Sherine M. Cruzate.
Laccase is a promising enzyme produce by wide range of organisms that has a potential application in industry, biotechnology, and treatment of various industrial wastes. Hence, this study aimed to screen and characterize laccase producing fungi from different industrial soil samples of Cavite. Screening of laccase producing fungi was done through colorimetric measurement using tannic acid and napthol as synthetic substrates. A total of 15 fungal isolates showed positive laccase production as indicated by the colored halo zones around their colonies when grown on PDA containing tannic acid. These isolates were identified belonging to five genera: Paecilomyces, Curvularia, Fusarium, Penicillium, Acremonium and five fungal isolates were not identified due to the variations in their morphotypes. Further screening test revealed that eleven and three isolates exhibited laccase production when grown on PDA with increasing concentrations of tannic acids and a-napthol, respectively. Among the laccase- producing fungi, DSYS2 was observed to be the most potent isolate when tannic acid was used as synthetic substrate while DPS6 was the only laccase producing fungi that can tolerate high concentrations of a —napthol. However, the produced colored zone was smaller compared to tannic acid. Only Paecilomyces spp. (DPS7) and DPS4 were able to produce laccase on both substrate indicators.
The results of this study confirmed the presence of laccase- producing fungi in the industrial soils of Cavite, and their laccase production varied depending on the substrate used.
Submitted to the University Library July 03, 2019 T-8070