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Screening of poly-β-hydroxybutyrate (PHB) - synthesizing bacteria isolated from various samples / by Alyssa Jane E. Cuadra.

By:

Cuadra, Alyssa Jane E [author]

Contributor(s):

Ilagan, Yolanda A [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2019.Description: xi, 69 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

589.3 C89 2019

Online resources:

Click here to view abstract and table of contents

Production credits:

College of Arts and Sciences (CAS), Department of Biological Sciences

Abstract: CUADRA, ALYSSA JANE E. Screening of Poly-B-hydroxybutyrate (PHB) - synthesizing Bacteria Isolated from Various Samples. Undergraduate Thesis, Bachelor of Science in Biology, Major in Microbiology, Cavite State University, Indang, Cavite. June 2019. Adviser: Dr. Yolanda A. Ilagan This study was conducted to isolate, screen, and identify bacterial isolates capable of producing poly-B-hydroxybutyrate (PHB) from various samples. It was conducted at the Microbiology Laboratory of the Research Center, Cavite State University, Indang Cavite from September 2018 to March 2019. Two hundred sixty-eight bacterial isolates were screened for possible PHB production using Sudan Black B plate assay. Results revealed that 20 bacterial isolates were found positive based on high color intensity, and detection of intracellular lipid inclusions by Sudan Black B Staining. The PHB producing isolates were characterized culturally, morphologically and physiologically to determine their identities at generic level. Following the scheme of identification, the isolates were presumptively identified under the genus Bacillus.

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Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	589.3 C89 2019 (Browse shelf(Opens below))		Available	T-8343		00079674

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Thesis (Bachelor of Science in Biology Major in Microbiology) Cavite State University.

Includes bibliographical references.

College of Arts and Sciences (CAS), Department of Biological Sciences

CUADRA, ALYSSA JANE E. Screening of Poly-B-hydroxybutyrate (PHB) - synthesizing Bacteria Isolated from Various Samples. Undergraduate Thesis, Bachelor of Science in Biology, Major in Microbiology, Cavite State University, Indang, Cavite. June 2019. Adviser: Dr. Yolanda A. Ilagan

This study was conducted to isolate, screen, and identify bacterial isolates capable of producing poly-B-hydroxybutyrate (PHB) from various samples. It was conducted at the Microbiology Laboratory of the Research Center, Cavite State University, Indang Cavite from September 2018 to March 2019.

Two hundred sixty-eight bacterial isolates were screened for possible PHB production using Sudan Black B plate assay. Results revealed that 20 bacterial isolates were found positive based on high color intensity, and detection of intracellular lipid inclusions by Sudan Black B Staining. The PHB producing isolates were characterized culturally, morphologically and physiologically to determine their identities at generic level. Following the scheme of identification, the isolates were presumptively identified under the genus Bacillus.

Submitted to the University Library 07/18/2019 T-8343