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Phytochemical analysis and evaluation of cytotoxicity and genotoxicity of breynia androgyna L. (Binahian) leaf extract / by Christian Joshua S. Costa.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2019.Description: xiv, 87 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 581.634  C82 2019
Online resources: Production credits:
  • College of Arts and Sciences (CAS), Department of Biological Sciences
Abstract: COSTA, CHRISTIAN JOSHUA S. Phytochemical Analysis and Evaluation of Cytotoxicity and Genotoxicity of Breynia androgyna L. (Binahian) Leaf Extract. Undergraduate Thesis. Bachelor of Science in Biology major in General Biology. Cavite State University, Indang, Cavite. June 2019. Adviser: Ms. Liwayway P. Taglinao. A study was conducted to assess the cytotoxicity and genotoxicity of Breynia androgyna L. (Binahian) ethanolic leaf extract using Allium cepa L. (Onion) test. Phytochemical analysis was performed on the leaf ethanolic extract of B. androgyna plant. In accordance, B. androgyna extract was diluted into different concentrations of plant extract as the treatment groups together with control groups. Six groups of onion bulbs were submerged into different treatment groups for two days and the morphological effects of the treatment were observed and documented. Onion roots were harvested and fixed for microscopic observation of the mitotic index and chromosomal aberrations. This study showed that the phytochemical compounds that were present on the B. adrogyna consist of tannins, flavonoids, phenolics, alkaloids and steroids. There is a significant difference on the onion root length when submerged to B. androgyna extract in comparison with the negative control group (p<0.05). Thus, there is a significant inhibition of mitotic activity in the onion group treated with plant extract compared to the negative control (p<0.05). On the other hand, the inhibition of the mitotic activity was highly significant in the 750 ug/mL and 1000 gg/mL B. androgyna plant extract. One thousand gg/mL B. androgyna plant extract decreases mitotic index at 32% thus signifying it as having sublethal effects on an organism. The number of chromosomal aberrations increases as the level of concentration of plant extract increases compared to the negative control (tapwater). There was statistically significant differences at all the values except for 250 pg/ml B. androgyna extract concentration and 500 gg/mL B.androgyna extract concentration (p<0.05).Hence, the leaves of B. androgyna are commonly utilized by human as food or medicine as revealed by the survey. However, B. androgyna plant leaves, if consumed or applied at certain doses (approximately beyond 500 gg/mL), will induce a cytotoxic and genotoxic effect on an organism
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 581.634 C82 2019 (Browse shelf(Opens below)) Link to resource Room use only T-8264 00079715

Thesis (Bachelor of Science in Biology Major in General Biology) Cavite State University.

Includes bibliographical references.

College of Arts and Sciences (CAS), Department of Biological Sciences

COSTA, CHRISTIAN JOSHUA S. Phytochemical Analysis and Evaluation of
Cytotoxicity and Genotoxicity of Breynia androgyna L. (Binahian) Leaf Extract.
Undergraduate Thesis. Bachelor of Science in Biology major in General Biology. Cavite
State University, Indang, Cavite. June 2019. Adviser: Ms. Liwayway P. Taglinao.

A study was conducted to assess the cytotoxicity and genotoxicity of Breynia androgyna
L. (Binahian) ethanolic leaf extract using Allium cepa L. (Onion) test. Phytochemical analysis
was performed on the leaf ethanolic extract of B. androgyna plant. In accordance, B. androgyna
extract was diluted into different concentrations of plant extract as the treatment groups together
with control groups. Six groups of onion bulbs were submerged into different treatment groups
for two days and the morphological effects of the treatment were observed and documented.
Onion roots were harvested and fixed for microscopic observation of the mitotic index and
chromosomal aberrations.

This study showed that the phytochemical compounds that were present on the B.
adrogyna consist of tannins, flavonoids, phenolics, alkaloids and steroids. There is a significant
difference on the onion root length when submerged to B. androgyna extract in comparison with
the negative control group (p<0.05). Thus, there is a significant inhibition of mitotic activity in the
onion group treated with plant extract compared to the negative control (p<0.05). On the other
hand, the inhibition of the mitotic activity was highly significant in the 750 ug/mL and 1000
gg/mL B. androgyna plant extract. One thousand gg/mL B. androgyna plant extract decreases
mitotic index at 32% thus signifying it as having sublethal effects on an organism. The number
of chromosomal aberrations increases as the level of concentration of plant extract increases
compared to the negative control (tapwater). There was statistically significant differences at all
the values except for 250 pg/ml B. androgyna extract concentration and 500 gg/mL
B.androgyna extract concentration (p<0.05).Hence, the leaves of B. androgyna are commonly
utilized by human as food or medicine as revealed by the survey. However, B. androgyna plant
leaves, if consumed or applied at certain doses (approximately beyond 500 gg/mL), will induce
a cytotoxic and genotoxic effect on an organism

Submitted to the University Library July 09, 2019 T-8264

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