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Antibacterial activity of selected tropical fruit leaf extracts against enterobacteriaceae / by Joey B. Asuncion, Jion P. Dimson, and Jansell Jane O. Reyes.

By:

Asuncion Joey B [author]

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2013.Description: xi, 70 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

615.329 As9 2013

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Nursing (CON)

Abstract: ASUNCION, JOEY B., DIMSON, JION P., AND REYES, JANSELL JANE O., Antibacterial Activity of Selected Tropical Fruit Leaf Extracts against Enterobacteriaceae. Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, April 2013. Adviser: Dr. Adelaida E. Sangalang This study was conducted from April to May 2012 at the Department of Medical Technology, College of Nursing, Cavite State University, Indang, Cavite. The study aimed to determine the antibacterial activity of selected tropical fruit leaf extracts against species of Enterobacteriaceae commonly associated with nosocomial infections. Fresh young tropical fruit leaves of avocado (Persea americana Mill.), guyabano (Annona muricata), mango (Mangifera indica L.), star apple (Chrysophyllum cainito), and sugar apple (Annona squamosa) were collected. The bioactive components of the leaves were extracted using hot water, cold water, and ethanol. Commercial bactericide ceftazidime and distilled water served as controls. The diameter of the zone of inhibition was measured after 24 hours of incubation using a ruler. Zones of inhibition were categorized as resistant, intermediate, and susceptible based on the Zone Diameter Interpretative Standards (ZDIS). The lowest effective concentration of the tropical fruit leaves was evaluated by determining the minimum inhibitory concentration (MIC) using broth dilution method. Results showed that the tropical fruit leaves extract exerted varying degrees of antibacterial activity towards the test organisms. Among these extracts, cold water extract of avocado and ethanol extracts of mango and star apple were the most effective against Escherichia coli. The hot water extract of mango, ethanol extract of star apple, and cold-water extract of sugar apple produced the highest zone of inhibition against Pseudomonas aeruginosa. Ethanol extract of avocado, hot water extract of guyabano and mango are the most effective against klebsiella pneumoniae. The minimum inhibitory concentration (MIC) of the tropical fruit leaf extracts were from 3.13 to 100 mg/ml. Ethanol proved to be the most effective solvent.

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Holdings ( 2 )
Title notes ( 6 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Copy number	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	615.329 As9 2013 (Browse shelf(Opens below))		Link to resource	c1	Room use only	T-4890		00000089
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	615.329 As9 2013 (Browse shelf(Opens below))		Link to resource	c2	Room use only	T-5153		00074574

Theses (BS Medical Technology) Cavite State University.

Includes bibliographical references.

College of Nursing (CON)

ASUNCION, JOEY B., DIMSON, JION P., AND REYES, JANSELL JANE O., Antibacterial Activity of Selected Tropical Fruit Leaf Extracts against Enterobacteriaceae. Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, April 2013. Adviser: Dr. Adelaida E. Sangalang

This study was conducted from April to May 2012 at the Department of Medical Technology, College of Nursing, Cavite State University, Indang, Cavite. The study aimed to determine the antibacterial activity of selected tropical fruit leaf extracts against species of Enterobacteriaceae commonly associated with nosocomial infections. Fresh young tropical fruit leaves of avocado (Persea americana Mill.), guyabano (Annona muricata), mango (Mangifera indica L.), star apple (Chrysophyllum cainito), and sugar apple (Annona squamosa) were collected. The bioactive components of the leaves were extracted using hot water, cold water, and ethanol. Commercial bactericide ceftazidime and distilled water served as controls. The diameter of the zone of inhibition was measured after 24 hours of incubation using a ruler. Zones of inhibition were categorized as resistant, intermediate, and susceptible based on the Zone Diameter Interpretative Standards (ZDIS). The lowest effective concentration of the tropical fruit leaves was evaluated by determining the minimum inhibitory concentration (MIC) using broth dilution method. Results showed that the tropical fruit leaves extract exerted varying degrees of antibacterial activity towards the test organisms. Among these extracts, cold water extract of avocado and ethanol extracts of mango and star apple were the most effective against Escherichia coli. The hot water extract of mango, ethanol extract of star apple, and cold-water extract of sugar apple produced the highest zone of inhibition against Pseudomonas aeruginosa. Ethanol extract of avocado, hot water extract of guyabano and mango are the most effective against klebsiella pneumoniae. The minimum inhibitory concentration (MIC) of the tropical fruit leaf extracts were from 3.13 to 100 mg/ml. Ethanol proved to be the most effective solvent.

Submitted to the University Library 8-4-2020 T-4890 copy 1

Submitted to the University Library 8-4-2020 T-5153 copy 2