Biological effects of dichloromethane leaf extract of bignai (Antidesma bunius) / by Gregory John B. Espidido.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2015. Cavite State University- Main Campus,Description: xv, 51 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 570  Es6 2015
Online resources: Production credits:
  • College of Veterinary Medicine and Biomedical Sciences (VETMET)
Abstract: ESPEDIDO, GREGORY JOHN B. Biological Effects of Dichloromethane Leaf Extract of Bignai (Antidesma bunius). Undergraduate Thesis, Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. April 2015. Adviser: Chester Joshua Saldana, DVM, MSc. A study was conducted to determine the biological activities of Antidesma bunius dichloromethane leaf extract. Its anti-inflammatory, insecticidal, cytotoxic and antimicrobial activities were assessed. The injection of carrageenan into the peritoneal cavity of mice induces an influx of cells and fluid accumulation with the appearance of neutrophils and lymphocytes. The White Blood Cell (WBC) count of Antidesma bunius leaf extract group was higher (8.7 x 103/0) than the positive control group (8.62 x 103411) and there was no significant difference between the two treatments. The mortality time lapse (MTL) of test ants after treatment with A. bunius leaf extract and controls revealed that exposure of ants to 1000 ppm A. bun/us leaf extract recorded MTL of 73.5 minutes and 109.5 minutes for 500 ppm which was relatively longer than those exposed to the commercial pesticide but significantly shorter than the negative control with 523.5 minutes. The Antidesma bunius leaf extract was able to exhibit cytotoxicity after obtaining maximum lethality of 100% at 10Oug/m1 and 1000ug/m1 concentrations and decreased at 10 ug/ml (50.00%), 5 [ig/m1 (12.50%) and 1 µg/m1 (0%), respectively. The lethal concentration 50 was established at 10µg/m1 (50%). On the other hand, Pseudomonas aeruginosa and Staphylococcus aureus displayed resistance to Antidesma bunius leaf extract but was less effective against Escherichia coli, Bacillus subtilis, Candida tropicalis and Aspergillus niger when compared to positive control. The results of this study showed that dichloromethane extract of Antidesma bunius leaf extract exhibit an anti-inflammatory, antibacterial, cytotoxic and insecticidal effect.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 570 Es6 2015 (Browse shelf(Opens below)) Link to resource Room use only T-5692 00009231

Thesis ( Doctor of Veterinary Medicine ) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

ESPEDIDO, GREGORY JOHN B. Biological Effects of Dichloromethane Leaf Extract of Bignai (Antidesma bunius). Undergraduate Thesis, Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. April 2015. Adviser: Chester Joshua Saldana, DVM, MSc.
A study was conducted to determine the biological activities of Antidesma bunius dichloromethane leaf extract. Its anti-inflammatory, insecticidal, cytotoxic and antimicrobial activities were assessed. The injection of carrageenan into the peritoneal cavity of mice induces an influx of cells and fluid accumulation with the appearance of neutrophils and lymphocytes. The White Blood Cell (WBC) count of Antidesma bunius leaf extract group was higher (8.7 x 103/0) than the positive control group (8.62 x 103411) and there was no significant difference between the two treatments. The mortality time lapse (MTL) of test ants after treatment with A. bunius leaf extract and controls revealed that exposure of ants to 1000 ppm A. bun/us leaf extract recorded MTL of 73.5 minutes and 109.5 minutes for 500 ppm which was relatively longer than those exposed to the commercial pesticide but significantly shorter than the negative control with 523.5 minutes. The Antidesma bunius leaf extract was able to exhibit cytotoxicity after obtaining maximum lethality of 100% at 10Oug/m1 and 1000ug/m1 concentrations and decreased at 10 ug/ml (50.00%), 5 [ig/m1 (12.50%) and 1 µg/m1 (0%), respectively. The lethal concentration 50 was established at 10µg/m1 (50%). On the other hand, Pseudomonas aeruginosa and Staphylococcus aureus displayed resistance to Antidesma bunius leaf extract but was less effective against Escherichia coli, Bacillus subtilis, Candida tropicalis and Aspergillus niger when compared to positive control. The results of this study showed that dichloromethane extract of Antidesma bunius leaf extract exhibit an anti-inflammatory, antibacterial, cytotoxic and insecticidal effect.

Submitted copy to the University Library. 06/02/2015 T-5692

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