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Surgical intensive care units (SICU) nosocomial pathogens among selected tertiary government hospital in Region IV-A / by Tricia Anne D. Malimban and Jilian Joi R. Manipis.

By:

Malimban, Tricia Anne D [author]

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University-Main Campus, 2016.Description: xiv, 84 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

362.1 M29 2016

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Nursing (CON)

Abstract: MALIMBAN, TRICCIA ANNE and MANIPIS, JILLIAN JOI. Surgical Intensive Care Units (SICU) Nosocomial Pathogens Among Selected Tertiary Government Hospitals in Region IV-A. Undergraduate Thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. April 2016. Adviser: Ms. Karen Krista Escobar, RMT, MSMT. The study was conducted from June 2015 to November 2015 in three government tertiary hospitals for the inoculum collection and in Cavite State University College of Nursing for the processing of specimens. Generally, it aimed to isolate and characterize phenotypically the nosocomial pathogens from surgical intensive care units of selected tertiary government hospitals in Region IV-A. Specifically, the study aimed to: (a) determine the phenotypic characteristics of nosocomial pathogens isolated from surgical intensive care units in selected tertiary hospitals; (b) identify the selected nosocomial pathogens present from surgical intensive care units in selected tertiary hospitals; and (c) determine the susceptibility levels of nosocomial pathogens isolated using disinfectants. This study used a descriptive research design for the determination of phenotypic characteristics. The methodology included selection of tertiary government hospitals, preparation of materials, sterilization of materials and media preparations, collection of samples, isolation of microorganisms, identification of potential pathogens in terms of physical characteristics, gram staining procedure and biochemical examination, standardization of bacterial suspension, and susceptibility testing. Three selected tertiary government hospitals in Region IV-A were selected for specimen collection, and designated into three trials with four replications (R). Frequency and percentage were used after the determination of nosocomial pathogens. Upon observation of the phenotypic characteristics such as physical characteristics on culture media, biochemical tests and gram staining results, four nosocomial pathogens were isolated. These pathogens were Citrobacter freundii, Enterobacter aerogenes, Salmonella spp. and Staphylococcus aureus. Citrobacter freundii, Enterobacter aerogenes, Salmonella spp., and Staphylococcus aureus were susceptible to Hydrogen peroxide. On the other hand, Citrobacter freundii and Enterobacter aerogenes showed an intermediate susceptibility level while Salmonella spp., and Staphylococcus aureus were susceptible in Sodium hypochlorite. In order to reduce the risk of nosocomial infections, the researchers recommend that healthcare personnel should be more careful and cautious of what they are handling before entering the surgical intensive care unit. It is also recommended to change sterile gloves after using as much as possible to avoid the spread of nosocomial infections. Re-orientation of the utility workers about standard cleaning procedures. Further research study related to isolation of nosocomial bacterial or fungal pathogens in fomites of surgical intensive care units or other special areas of the hospital should be conducted. Use selective media in order to easily identify the different nosocomial pathogens present. Isolation of nosocomial pathogens from different tubing such as endotracheal tube, catheter tubes and nasogastric tubes are recommended, too.

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Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	362.1 M29 2016 (Browse shelf(Opens below))		Link to resource	Room use only	T-5890		00009620

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

MALIMBAN, TRICCIA ANNE and MANIPIS, JILLIAN JOI. Surgical Intensive Care Units (SICU) Nosocomial Pathogens Among Selected Tertiary Government Hospitals
in Region IV-A. Undergraduate Thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. April 2016. Adviser: Ms. Karen Krista Escobar, RMT, MSMT.

The study was conducted from June 2015 to November 2015 in three government tertiary hospitals for the inoculum collection and in Cavite State University College of Nursing for the processing of specimens. Generally, it aimed to isolate and characterize phenotypically the nosocomial pathogens from surgical intensive care units of selected tertiary government hospitals in Region IV-A. Specifically, the study aimed to: (a) determine the phenotypic characteristics of nosocomial pathogens isolated from surgical intensive care units in selected tertiary hospitals; (b) identify the selected nosocomial pathogens present from surgical intensive care units in selected tertiary hospitals; and (c) determine the susceptibility levels of nosocomial
pathogens isolated using disinfectants.

This study used a descriptive research design for the determination of phenotypic characteristics. The methodology included selection of tertiary government hospitals, preparation of materials, sterilization of materials and media preparations, collection of samples, isolation of microorganisms, identification of potential pathogens in terms of physical characteristics, gram staining procedure and biochemical examination, standardization of bacterial suspension, and susceptibility testing. Three selected tertiary government hospitals in Region IV-A were selected for specimen collection, and designated into three trials with four replications (R). Frequency and percentage were used after the determination of nosocomial pathogens.

Upon observation of the phenotypic characteristics such as physical characteristics on culture media, biochemical tests and gram staining results, four nosocomial pathogens were isolated. These pathogens were Citrobacter freundii, Enterobacter aerogenes, Salmonella spp. and Staphylococcus aureus. Citrobacter freundii, Enterobacter aerogenes, Salmonella spp., and Staphylococcus aureus were susceptible to Hydrogen peroxide. On the other hand, Citrobacter freundii and Enterobacter aerogenes showed an intermediate susceptibility level while Salmonella spp., and Staphylococcus aureus were susceptible in Sodium hypochlorite.

In order to reduce the risk of nosocomial infections, the researchers recommend that healthcare personnel should be more careful and cautious of what they are handling before entering the surgical intensive care unit. It is also recommended to change sterile gloves after using as much as possible to avoid the spread of nosocomial infections. Re-orientation of the utility workers about standard cleaning procedures. Further research study related to isolation of nosocomial bacterial or fungal pathogens in fomites of surgical intensive care units or other special areas of the hospital should be conducted. Use selective media in order to easily identify the different nosocomial pathogens present. Isolation of nosocomial pathogens from different tubing such as endotracheal tube, catheter tubes and nasogastric tubes are recommended, too.

Submitted to the University Library 08/04/2020 T-5890