Antifungal effect of selected plant methanolic extracts against Candida albicans and Fusarium oxysporum / by Mark Mhardhin M. Francia, Marilyn L. Mojica and Jethro L. Piores.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : Cavite State University-Main Campus, 2015.Description: xiv, 74 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 579.5 F84 2015
Online resources: Production credits:
  • College of Nursing (CON)
Abstract: FRANCIA, MARK MHARDHIN M., MOJICA, MARLYN L., & PIORES, JETHRO L. Antifungal Effect of Selected Plant Methanolic Extracts Against Candida albicans and Fusarium oxysporum. Undergraduate Thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. October 2015. Adviser: Dr. Adelaida E. Sangalang. The study was conducted at the National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Banos from April to June 2014. The study generally aimed to compare the levels of antifungal activity of six selected leaf extracts of: Mangifera indica (mango), Averrhoa bilimbi (kamias), Psidium guajava (guava), Artocarpus heterophyllus (jackfruit), Annona muricata (soursop), and Carica papaya (papaya) against Candida albicans and Fusarium oxysporum. Plant leaf samples were sent to the University of the Philippines Los Banos Herbarium for authentication. The samples were air dried, ground and extracted using methanol. Candida albicans and Fusarium oxysporum were procured from Philippine National Collection of Microorganisms (PNCM) at the National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Banos. Antimicrobial assay was done by cylinder-cup technique and results were obtained by measuring the zone of inhibition (mm) after 2 days and 4 days of incubation for Candida albicans and Fusarium oxysporum, respectively. The methanolic leaf extracts of guava and mango exhibited high antifungal activity against Candida albicans. On the other hand, kamias, jackfruit, soursop and papaya have a low antifungal activity. Guava and mango leaf extracts were significantly more effective compared to the four leaf extracts. These plant extracts contained a lot of active antifungal component, such as, tannins, flavonoid, terpenoids and glycosides. Fusarium oxysporum showed susceptibility to the fungicide used but yielded resistance to the six plant methanolic extracts. The results indicate that the substrate in which each test fungus cleaved different results. C. albicans was significantly inhibited by guava and mango extracts. The extracts can be used as an alternative for the commercial fungicide, such as itraconazole. However, Fusarium oxysporum is resistant to all the six methanolic leaf extracts.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 579.5 F84 2015 (Browse shelf(Opens below)) Link to resource Room use only T-5896 00009621

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

FRANCIA, MARK MHARDHIN M., MOJICA, MARLYN L., & PIORES, JETHRO L. Antifungal Effect of Selected Plant Methanolic Extracts Against Candida albicans and Fusarium oxysporum. Undergraduate Thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. October 2015. Adviser: Dr. Adelaida E. Sangalang.

The study was conducted at the National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Banos from April to June 2014. The study generally aimed to compare the levels of antifungal activity of six selected leaf extracts of: Mangifera indica (mango), Averrhoa bilimbi (kamias), Psidium guajava (guava), Artocarpus heterophyllus (jackfruit), Annona muricata (soursop), and Carica papaya (papaya) against Candida albicans and Fusarium oxysporum.

Plant leaf samples were sent to the University of the Philippines Los Banos Herbarium for authentication. The samples were air dried, ground and extracted using methanol. Candida albicans and Fusarium oxysporum were procured from Philippine National Collection of Microorganisms (PNCM) at the National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Banos. Antimicrobial assay was done by cylinder-cup technique and results were obtained by measuring the zone of inhibition (mm) after 2 days and 4 days of incubation for Candida albicans and Fusarium oxysporum, respectively.

The methanolic leaf extracts of guava and mango exhibited high antifungal activity against Candida albicans. On the other hand, kamias, jackfruit, soursop and papaya have a low antifungal activity. Guava and mango leaf extracts were significantly more effective compared to the four leaf extracts. These plant extracts contained a lot of active antifungal component, such as, tannins, flavonoid, terpenoids and glycosides. Fusarium oxysporum showed susceptibility to the fungicide used but yielded resistance to the six plant methanolic extracts. The results indicate that the substrate in which each test fungus cleaved different results. C. albicans was significantly inhibited by guava and mango extracts. The extracts can be used as an alternative for the commercial fungicide, such as itraconazole. However, Fusarium oxysporum is resistant to all the six methanolic leaf extracts.

Submitted to the University Library 08/04/2020 T-5896

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