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Pathogenic waterborne bacteria of water sources in Naic, Cavite / by Nirvia G. Mila.

By:

Mila, Nirvia G [author]

Contributor(s):

De Guzman, Flordeliza A [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University-Main Campus, 2016.Description: xv, 72 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

579.3 M58 2016

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Nursing (CON)

Abstract: MILA, NIRVIA G., SALVACION, JESUSA A. Pathogenic Waterborne Bacteria of Water Sources in Naic, Cavite. Undergraduate Thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. January 2016. Adviser: Flordeliza A. De Guzman RMT, MAT. This study entitled "Pathogenic Waterborne Bacteria of Water Sources in Naic, Cavite" was performed between August to November 2015 that aimed to: (1) isolate waterborne bacteria in Naic, Cavite in the water resources namely hand pump (poso), commercial water (water station) and pressured-pump (private sources); (2) characterize whether the isolated sample has non-pathogenic or pathogenic waterborne bacteria through colony physical characteristics (in selective agar: Mannitol Salt Agar, Xylose-Lysine Deoxycholate Agar, and Thiosulfate Citrate Bile-Salt Sucrose Agar) and gram staining; (3) correlate the standard colony count to the actual count of bacterial colony of the identified pathogen in analyzing if it can induce disease; and (4) compare the three water sources according to the presence of isolated pathogenic waterborne bacteria. The water samples from different water sources in the municipality were randomly collected and were subjected to serial dilution, culture on different selective media for identification of specific waterborne bacteria based on colony morphology, gram staining for microscopic examination to identify whether the bacteria were gram-negative or gram-positive and colony count to identify whether the bacteria were pathogenic or non-pathogenic. The study showed that the water sources is capable of harboring pathogenic waterborne bacteria, such as Escherichia coli, Shigella dysenteriae, Salmonella enterica, Vibrio cholerae, and Staphylococcus aureus, with highest number of isolates from hand-pump water, followed by pressured-pump and commercial water. It is recommended that lined-water sources in the municipality be included in the further researches to confirm possible occurrence of pathogenic waterborne bacteria.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	579.3 M58 2016 (Browse shelf(Opens below))		Link to resource	Room use only	T-5891		00009692

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

MILA, NIRVIA G., SALVACION, JESUSA A. Pathogenic Waterborne Bacteria of Water Sources in Naic, Cavite. Undergraduate Thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. January 2016. Adviser: Flordeliza A. De Guzman RMT, MAT.

This study entitled "Pathogenic Waterborne Bacteria of Water Sources in Naic, Cavite" was performed between August to November 2015 that aimed to: (1) isolate waterborne bacteria in Naic, Cavite in the water resources namely hand pump (poso), commercial water (water station) and pressured-pump (private sources); (2) characterize whether the isolated sample has non-pathogenic or pathogenic waterborne bacteria through colony physical characteristics (in selective agar: Mannitol Salt Agar, Xylose-Lysine Deoxycholate Agar, and Thiosulfate Citrate Bile-Salt Sucrose Agar) and gram staining; (3) correlate the standard colony count to the actual count of bacterial colony of the identified pathogen in analyzing if it can induce disease; and (4) compare the three water sources according to the presence of isolated pathogenic waterborne bacteria.

The water samples from different water sources in the municipality were randomly collected and were subjected to serial dilution, culture on different selective media for identification of specific waterborne bacteria based on colony morphology, gram staining for microscopic examination to identify whether the bacteria were gram-negative or gram-positive and colony count to identify whether the bacteria were pathogenic or non-pathogenic. The study showed that the water sources is capable of harboring pathogenic waterborne bacteria, such as Escherichia coli, Shigella dysenteriae, Salmonella enterica, Vibrio cholerae, and Staphylococcus aureus, with highest number of isolates from hand-pump water, followed by pressured-pump and commercial water. It is recommended that lined-water sources in the municipality be included in the further researches to confirm possible occurrence of pathogenic waterborne bacteria.

Submitted to the University Library 08/04/2020 T-5891