Screening of the anticoagulant property of cogon grass (imperata cylindrica) extracts / by Aeron Mark P. Hernando and Ryan Alexander B. Pelayo.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2016. Cavite State University- Main Campus,Description: xv, 44 pages : 28 cm. illustrations ; Content type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 584  H43 2016
Online resources: Production credits:
  • Science High School, College of Education (CED)
Abstract: HERNANDO, AERON MARK P. asd PELAYO, RYAN ALEXANDER B. Screening of the Anticoagulant Property of Cogon Grass (Imperata cylindrica) Extracts. Research Study. Science High School (General Science Curriculum), College of Education, Cavite State University, Indang, Cavite. April 2016. Adviser. Ms. Karen Krista M. Escobar. The study was conducted from December 2015 to March 2016 at the Department of Biological Sciences, College of Arts and Sciences, Cavite State University, Indang, Cavite, to screen the anticoagulant property of cogon grass (Imperata cylindrica) extracts. Specifically, it aimed to: determine the solvent that would yield the highest amount of cogon grass extract, determine the phytochemicals present in the cogon grass extracts, determine the anticoagulant activity of the cogon grass extracts with different polarities in terms of prothrombin time and the partial thromboplastin time; and identify the significant difference in the anticoagulant activity among the cogon grass with different polarities. There were five treatments with three replications in the study. The five treatments used were as follow. Ty (Negative control), T, (Positive control); T>, (Ethanoic extract); T; (Dichloroethanoic extract); and T, (Hexanolic extract). The collection and drying of cogon grass were conducted from December 2015 — January 2016. After drying, the leaves were pulverised into fine-like powder and extracts were evaluated. The produced extracts from different solvents were used as treatments. The phytochemical screening, and prothrombin and partial thromboplastin time tests were conducted from February to March 2016. The amount of extracts produced from cogon grass was determined and based on the results, hexane (E3) had the highest volume of yielded extract while ethanol (E,) had the least yielded extract. The phytochemicals present in each extract were also determined and it showed that ethanolic extracts had the presence of tannins, phenols, and terpenoids. Dichloromethane extracts showed the presence of tannins and flavonoids. Hexane extract showed the presence of tannins, phenols and alkaloids. The prothrombin and partial thromboplastin time of the five treatments were determined. The PT test results showed that the treatment with the longest coagulation time was T2 (Serum + Ethanolic extract), making it the best of all the treatments with cogon extracts. It gave a mean of 219.33 seconds. On the other hand, PTT test results showed that the treatment with the longest coagulation time was T, (Serum + Hexane extract) with a mean of 213.33 seconds. But T, (Positive control) still had the longest coagulation time in both PT and PTT test results and are comparable to the treatments with cogon extracts. Other treatments except Ty (Positive control) also showed better results.
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Research Study (Science High School) Cavite State University

Includes bibliographical references.

Science High School, College of Education (CED)

HERNANDO, AERON MARK P. asd PELAYO, RYAN ALEXANDER B. Screening of the Anticoagulant Property of Cogon Grass (Imperata cylindrica) Extracts. Research Study. Science High School (General Science Curriculum), College of Education, Cavite State University, Indang, Cavite. April 2016. Adviser. Ms. Karen Krista M. Escobar.

The study was conducted from December 2015 to March 2016 at the Department of Biological Sciences, College of Arts and Sciences, Cavite State University, Indang, Cavite, to screen the anticoagulant property of cogon grass (Imperata cylindrica) extracts. Specifically, it aimed to: determine the solvent that would yield the highest amount of cogon grass extract, determine the phytochemicals present in the cogon grass extracts, determine the anticoagulant activity of the cogon grass extracts with different polarities in terms of prothrombin time and the partial thromboplastin time; and identify the significant difference in the anticoagulant activity among the cogon grass with different polarities.

There were five treatments with three replications in the study. The five treatments used were as follow. Ty (Negative control), T, (Positive control); T>, (Ethanoic extract); T; (Dichloroethanoic extract); and T, (Hexanolic extract).

The collection and drying of cogon grass were conducted from December 2015 — January 2016. After drying, the leaves were pulverised into fine-like powder and extracts were evaluated. The produced extracts from different solvents were used as treatments. The phytochemical screening, and prothrombin and partial thromboplastin time tests were conducted from February to March 2016.

The amount of extracts produced from cogon grass was determined and based on the results, hexane (E3) had the highest volume of yielded extract while ethanol (E,) had the least yielded extract. The phytochemicals present in each extract were also determined and it showed that ethanolic extracts had the presence of tannins, phenols, and terpenoids. Dichloromethane extracts showed the presence of tannins and flavonoids. Hexane extract showed the presence of tannins, phenols and alkaloids.

The prothrombin and partial thromboplastin time of the five treatments were determined. The PT test results showed that the treatment with the longest coagulation time was T2 (Serum + Ethanolic extract), making it the best of all the treatments with cogon extracts. It gave a mean of 219.33 seconds. On the other hand, PTT test results showed that the treatment with the longest coagulation time was T, (Serum + Hexane extract) with a mean of 213.33 seconds. But T, (Positive control) still had the longest coagulation time in both PT and PTT test results and are comparable to the treatments with cogon extracts. Other treatments except Ty (Positive control) also showed better results.

Submitted copy to the University Library. 10/11/2016 RS-760

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