Dimasacat, Mylord D. <br/><br/>
Detection of mt-rs s gene and flavivirus in ticks from cattle in the province of Cavite using polymerase chain reaction / 
by Mylord D. Dimasacat. 
 - Indang, Cavite :  Cavite State University- Main Campus,  2017. 
 - xiii, 59 pages :  illustrations ;	  28 cm. 
<br/><br/> Thesis (Doctor of Veterinary Medicine) Cavite State University 
<br/><br/>Includes bibliographical references. 
<br/><br/>College of Veterinary Medicine and Biomedical Sciences (VETMET)  College of Veterinary Medicine and Biomedical Sciences (VETMET) 
<br/><br/> 		DIMASACAT, MYLORD DIMAANO., Detection of mt-rrs gene and Flavivirus in ticks from cattle in the province of Cavite using Polymerase Chain Reaction Cavite Undergraduate Thesis, Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. April 2017. Adviser: Dr. Cherry R Alvarez.<br/><br/>The study was conducted to detect the presence of mt-rrs gene and Flavivirus from the cattle in the selected farms of the central hilly and lowland areas in the province of Cavite. Manual picking of ticks was performed in the cattle's body surface while flagging and dragging methods were employed in the pasture land. A total of one hundred (100) ticks were selected and used for the DNA/RNA extraction. The cDNA was synthesized using the ReverTra Ace-u-@ and were initially tested for the presence of mtrrs gene through the conventional Polymerase Chain Reaction (PCR).<br/>Of the eighty-nine (89) remaining samples, eighty (80) samples were positive for mt-rrs gene. This is comprised of eight (8) adult male ticks and seventy-two (72) adult feinale ticks, with a detection rate of 88.88% and 90.00%, respectively.<br/>All the mt-rrs positive samples were subjected to the detection of Flavivirus using Langat virus as positive control through conventional PCR but no gene was amplified. The results of this study suggested that the tick-borne Flaviviruses may not be present in the areas where the samples were collected.<br/>The author recommends that further study be conducted with a higher sample size, with nymph and larval stages of ticks as samples may also be included. Furthermore, pooling of samples may be considered to increase the possibility of detecting the pathogen by the number of ticks per pool. Lastly, inclusion of other<br/>xii<br/>animals, small ruminants, for instance, may be considered as sample animals <br/> 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Ticks
<br/><br/><label>Dewey Class. No.: </label>595.4   / D59 2017