Generoso, Florante Homoroc <br/><br/>
Detection of mt-rss gene and Coxiella burnetii among ticks collected from cattle and pasture in Cavite using polymerase chain reaction / 
by Florante Homoroc Generoso. 
 - Indang, Cavite :  Cavite State University- Main Campus,  2017. 
 - xivl, 62 pages :  illustrations ;	  28 cm. 
<br/><br/> Thesis (Doctor of Veterinary Medicine) Cavite State University 
<br/><br/>Includes bibliographical references. 
<br/><br/>College of Veterinary Medicine and Biomedical Sciences (VETMET)  College of Veterinary Medicine and Biomedical Sciences (VETMET) 
<br/><br/> FLORANTE H. GENEROSO, DETECTION OF mt-rrs GENE and Coxiella burnetii AMONG TICKS COLLECTED FROM CATTLE AND PASTURE IN CAVITE USING POLYMERASE CHAIN REACTION, Undergraduate Thesis. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite, May 2017, Adviser: Noemi D. Encarnacion, DVM, MVetEpi<br/>Ticks are considered vectors of pathogens for animals and humans. They are<br/>obligate ectoparasites that feed on blood tissues of wide host range. This study aimed to determine the presence of mt-rrs gene and Coxiella burnetti among tick samples from cattle farms within the province of Cavite, Philippines using polymerase chain reaction.<br/>A total 294 tick samples, 63 from lowland (21.42%) and 231 from central hilly (78.57%) were used in this study. All are of Rhipicephalus microplus species and comprises of 293 adults (99.65%) and I larva (0.35%). Ticks among the adult stage<br/>consist of 18 males (6.14%) and 275 females (93.85%).<br/>Out of 294 samples, 205 tick DNA extracted using the boiling method detected 85<br/>mt-rrs gene while using the BlackPREP Tick DNA/RNA kit (Life Science, Germany),<br/>out of the 89 tick DNA extracted, mt-rrs was detected in 80 samples. One hundred sixtyfive (165) tick DNA samples were ran for pathogen detection using Coxiel/a-specific<br/>primers. Zero (0) out of 165 tick DNA samples tested positive for the presence of Coxiella burnetii. One (1) sample on the other hand, produced a single, clear, but having<br/>a smaller amplicon suggesting the presence of Coxie//a-like endosymbiont in the tick<br/>POPLIIation. However, phylogenetic analysis is needed to support such claim.<br/>This study documents the detection rate of the mt-rrs gene using the boiling method and BlackPREP Tick DNA/RNA kit, (Life Science, Germany); and preliminary molecular survey of Coxiella burnetii from cattle ticks in Cavite.<br/> 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Ticks<br/>Cattle<br/>Pastures
<br/><br/><label>Dewey Class. No.: </label>595.4   / G28 2017