Identification of common medically important microbial isolates and the sanitation techniques of selected lying-In clinics in Cavite /
by Althea M. Arayata, Chris John M. Manio, and Neomi Gabrielle P. Nazareno.
- Indang, Cavite : Cavite State University- Main Campus, 2018.
- xvii, 124 pages : illustrations ; 28 cm.
Research Study (Bachelor of Science in Medical Technology) Cavite State University
Includes bibliographical references.
College of Nursing (CON) College of Nursing (CON)
ARAYATA, ALTEA M., MANIO, CHRIS JOHN N. AND NAZARENO, NEOMI GABRIELLE P. Identification of Common Medically Important Microbial Isolates and the Sanitation Techniques of Selected Lying-In Clinics in Cavite. An Undergraduate Research Study, Department of Medical Technology, College of Nursing, Cavite State University Indang, Cavite September 2017. Adviser: Bernadette S. Sapinoso, Ph.D. Co-Adviser: Hazel Joyce L. Guiao, RMT The study was conducted from July 2017 to September 2017. The samples were acquired in selected five lying-in clinics in Cavite, and were processed at the Biological Sciences Laboratory of Cavite State University. This study generally intended to isolate and identify bacteria as well as to evaluate the sanitary techniques of the selected lying-in clinics in Cavite. This study used a descriptive research design for the determination of phenotypic and biochemical characteristics of the isolates as well as the sanitation techniques. The materials needed for the experiment were bought and prepared before the researchers proceeded with the two methods of sample collection; bacterial swab from fomites and settle plate method. There were thirteen bacteria identified on the five selected lying-in clinics. Eleven of them were (a) pathogenic and opportunistic (Citrobacter, Enterobacter, Escherichia, Hafnia, Klebsiella, Providencia, Pseudomonas, Salmonellla, Serratia, Shigella, and Staphylococcus aureu.v), while two were considered (b) non-pathogenic (Bacillus and Coagulase Negative Staphylococcus). The sanitation techniques of the lying-in clinics were determined based on their sterilization (incineration, autoclave, dry heat, filtration, ionizing radiation, and chemicals), disinfection (boiling of water, UV light, bleach, alcohol, chlorine dioxide, hydrogen peroxide, glutaraldehyde, formaldehyde, and phenol), handwashing, clinical staff practices (use of slippers, eating, change of bed linens, and use of sterile materials) and healthcare waste management (placenta pit and chemical disinfectant).