Cytotoxic activity of crude aqueous leaf extracts of bignay (Antidesma nunius), alagaw (premna odorata) and serpentine (Rauvolfia serpentine) using brine shrimp lethality assay /
by Laurent Lee M. Magat, Cyrenne A. Vicedo, and Carlos Joaquin A. Mojica.
- Indang, Cavite : Cavite State University- Main Campus, 2018.
- xii, 57 pages : illustrations ; 28 cm.
Research Study (Doctor of Veterinary Medicine) Cavite State University.
Includes bibliographical references.
College of Veterinary Medicine and Biomedical Sciences (CVMBS) College of Veterinary Medicine and Biomedical Sciences (CVMBS)
MAGAT, LAURENT M., MOJICA, CARLOS JOAQUIN A., and VICEDO, CYRENNE A., Cytotoxic Activity Of Crude Aqueous Leaf Extracts Of Bignay (Antidesma Bunius), Alagaw (Premna Odorata) and Serpentina (Rauvolfia Serpentina) Using Brine Shrimp Lethality Assay. Research Study (General Science Curriculum). Science High School, College of Education, Cavite State University, Indang, Cavite. May 2018. Adviser: Mr Lloyd 0. Balinado This study was conducted to evaluate the toxicity of crude aqueous leaf extracts of Bignay (Antidesma bunius), Alagaw (Premna odorata), and Serpentina (Rauvolfia serpentina) The study specifically aimed to: determine the percentage mortalities of brine shrimp nauplii exposed to varying concentrations of crude aqueous leaf extracts of A. bunius, P. odorata and R. serpentina; determine the median lethal time of every plant extract concentration that could kill 50 percent of the brine shrimp population; determine the median lethal concentration (LC50) of each plant extract that can kill 50 percent of the brine shrimp population; determine the level of toxicity of the plant extracts based on their LC50 values. Each leaf extracts of Antidesma bunius, Premna odorata and Rauvolfia serpentina were prepared in 1 mg/mL, 0.50 mg/mL, 0.25 mg/mL 0.10 mg/mL and 0.05 mg/mL concentrations and were screened for cytotox city potential using Brine Shrimp Lethality Assay (BSLA). Cytotox city was evaluated in terms of mortality rates from where median lethal concentration (LC50) and median lethal time (LT50) were derived. Results of the study showed that the 0.05 mg/mL concentration of Premna odorata and Rauvofa serpentina leaf extract and 0.10 mg/mL concentration of Antidesma bunius showed the greatest cytotoxic effect against brine shrimp nauplii. The LC5o value of Antidesma bunius was 19.86 mg/mL, Premna odorata was 9.78 mg/mL, and 1auvolfia cetpenti a was 15.03mg/mL. Results indicated that the three leaf extracts were non-toxic based on Clarkson's toxicity criterion. Therefore, this may serve as baseline information for future medical research.