Isolation and identification of endophytic and epiphytic bacteria from kaong (Arenga pinnata (Wurmb) Merr.) leaves with potential antimicrobial activity /
by Jennlyn P. Abinal.
- Indang, Cavite : Cavite State University- Main Campus, 2019.
- xiii, 81 pages : illustrations ; 28 cm.
Thesis (Bachelor of Science in Biology Major in Microbiology) Cavite State University.
Includes bibliographical references.
College of Arts and Sciences (CAS), Department of Biological Sciences. College of Arts and Sciences (CAS), Department of Biological Sciences.
ABINAL, JENNLYN P. Isolation and Identification of Endophytic and Epiphytic Bacteria from Kaong (Arenga pinnata (Wurmb) Merr.) Leaves with Potential Antimicrobial Activity. An Undergraduate thesis. Bachelor of Science in Biology, Cavite State University, Indang, Cavite. June 2019. Adviser: Ms. Jamaica Q. Espineli A study was conducted to isolate endophytic and epiphytic bacteria from kaong leaves, determine their potential antimicrobial activity and identify the epiphytic and endophytic strains with potential antimicrobial activity. Leaf samples were gathered from six selected barangays in the town of Silang, and Indang, Cavite. Isolates obtained from the leaf samples were tested for the antagonistic effect against five bacterial strains Escherichia coli BA 1825, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 12600, Micrococcus luteus ATCC 10240, Bacillus subtilis B1514 and three fungal strains Aspergillus brasiliensis ATCC 16404, Candida albicans ATCC 10231, and Fusarium oxysporum NGS through cross streak assay. The isolates with antimicrobial activity were subjected to morphological and biochemical tests for identification. A total of 136 epiphytic and endophytic bacteria were isolated from the kaong leaves, 98 (72%) were epiphytic while 38 (28%) were endophytic. Among these isolates, only 32 (4 endophytes, 28 epiphytes) showed antimicrobial activities. Only one isolate under the genus Pseudomonas inhibited the two test organisms, S. aureus and M luteus. Isolated bacteria with potential antimicrobial activities were presumptively identified to the genera Pseudomonas, Bacillus, Aeromonas and Enterobacter.