Freezability of Murrah bull semen in different extenders /
by Kustono.
- Los Ba�os, Laguna, Cavite State University- Main Campus, 1985.
- 77 pages : illustrations ; 28 cm.
Thesis (M.S.--Animal Science) University of the Philippines
Includes bibliographical references.
College of Agriculture, Food, Environment and Natural Resources (CAFENR) College of Agriculture, Food, Environment and Natural Resources (CAFENR)
KUSTONO, University of the Philippines at Los Banos, May, 1985. Freezability of Murrah Bull Semen in Different Extenders. Major Professor: Prof. Orlando A. Palad The freezability of Murrah bull semen in Tris-egg-yolk-citrate-glycerol (TYG), skimmilk egg-yolk-citrate glycerol (SM-ECG) and glycine-egg-yolk-citrate glycerol (Gly-ECG) extenders using 1, 2 and 3 hours of equilibration time were studied. Extended semen were packed in 0.5 ml French straws, frozen in liquid nitrogen (-1960C) and stored for a period of 5 months. Post-thawing motility of sperm 15 minutes after freezing were 31.8, 28.6 and 27.7 percent in extender TYG, SM-ECG and Gly-ECG, respectively. Extended semen equilibrated for 1, 2, and 3 hours had, an average post-thawing motility of 23.0, 31.6 and 33.5 percent, respectively. The average post-thawing motility for Bull 1 and Bull 2 were 28.64 and 30.1 percent, respectively. Highly significant (P40.01) differences were found among treatments. Likewise a highly significant (P .C. 0.01) bull x extender interaction just after freezing on post-freezing motility was obtained. Differences in post-thawing motility among the treatments were highly significant (P(0.01) during the storage period of five months. Likewise, highly significant (P <0.01) interactions were observed on the following: bull x extender and bull x equilibration time during the first month of storage; bull x extender and extender x equilibration time during the second month of storage; and extender x equilibration time during the fourth month storage period. From the results of the study, it may be concluded that TYG is superior to both SM-ECG and Gly-ECG in terms of post-thawing motility provided that high quality semen should be used and that proper handling of the semen must be done until they are frozen. Along with the above suggestions, it is further recommended that the extended semen which will be deep-frozen should undergo an equilibration period of not less than three hours.