Antibiotic sensitivity profile of Salmonella spp. isolates in the spleen of chicken (Gallus gallus domesticus) from a dressing plant at Trece Martires City, Cavite
by Mervyn A. Mojica
- Indang, Cavite : Cavite State University- Main Campus, 2008.
- xv, 66 pages : illustrations ; 28 cm.
Thesis (Doctor of Veterinary Medicine) Cavite State University
Includes bibliographical references.
College of Veterinary Medicine and Biomedical Sciences (VETMET) College of Veterinary Medicine and Biomedical Sciences (VETMET)
MOJICA MERVYN ATAS, April 2008 Cavite State University, Indang, Cavite. Antibiotic Sensitivity Profile of Salmonella spp. Isolate in the Spleen of Chickens (Gallus gallus domesticus) from a Dressing Plant at Trece Martires City, Cavite. Adviser: Dr. Ma. Cynthia R. dela Cruz.
The study was conducted to isolate, characterize and evaluate the antiobiotic sensitivity profile of Salmonella spp. isolate in the spleen of chickens in a dressing plant at Trece Martires City, Cavite.
A total of fifty presumptive Salmonella spp. characterised as red colonies with or without black centres were isolated from one hundred fifty spleen samples in Xylose\ Lysine Desoxycholate Agar. The isolates were further subjected to morphological, biochemical and serological characterization and one isolate was identified as Salmonella spp. with the following characteristics: gram-negative rods on gram staining; Alk/A, H2S in Triple Sugar Iron Test, citrate positive, indole negative, hydrogen sulphide formation positive, motile, Methyl Red-Vogues Proskauer Test positive-negative, gelatinase negative, urease negative, and nitrate positive on biochemical test; and produced positive agglutination result on Salmonella O and Vi antigens. The antibiotic sensitivity profile of the isolate revealed that the isolate was sensitive to gentamicin, trimethoprim- sulfonamide, cephalothin, norfloxacin, ampicillin, and fosfomycin but resistant to lincomycin, tetracycline, and erythromycin. One colony isolated was confirmed to be Salmonella spp. Results of the study gave a prevalence rate of 0.67%, similar with the findings abroad wherein the spleen has the lowest prevalence of Salmonella spp.
The author recommends that molecular characterization be conducted to determine the prevalent serotype of Salmonella present in the animals sampled. Moreover, the author recommends testing of other antibiotics especially fluoroquinolones and chloramphenicol, because Salmonella was reported to be producing resistance to this family of antibiotics.