Asuncion Joey B. <br/><br/>
Antibacterial activity of selected tropical fruit leaf extracts against enterobacteriaceae / 
by Joey B. Asuncion, Jion P. Dimson, and Jansell Jane O. Reyes. 
 - Indang, Cavite :  Cavite State University- Main Campus,  2013. 
 - xi, 70 pages :  illustrations ;  28 cm. 
<br/><br/> Theses (BS Medical Technology) Cavite State University. 
<br/><br/>Includes bibliographical references. 
<br/><br/>College of Nursing (CON)  College of Nursing (CON) 
<br/><br/> ASUNCION, JOEY B., DIMSON, JION P., AND REYES, JANSELL JANE O., Antibacterial Activity of Selected Tropical Fruit Leaf Extracts against Enterobacteriaceae. Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, April 2013. Adviser: Dr. Adelaida E. Sangalang <br/><br/>This study was conducted from April to May 2012 at the Department of Medical Technology, College of Nursing, Cavite State University, Indang, Cavite. The study aimed to determine the antibacterial activity of selected tropical fruit leaf extracts against species of Enterobacteriaceae commonly associated with nosocomial infections. Fresh young tropical fruit leaves of avocado (Persea americana Mill.), guyabano (Annona muricata), mango (Mangifera indica L.), star apple (Chrysophyllum cainito), and sugar apple (Annona squamosa) were collected. The bioactive components of the leaves were extracted using hot water, cold water, and ethanol. Commercial bactericide ceftazidime and distilled water served as controls. The diameter of the zone of inhibition was measured after 24 hours of incubation using a ruler. Zones of inhibition were categorized as resistant, intermediate, and susceptible based on the Zone Diameter Interpretative Standards (ZDIS). The lowest effective concentration of the tropical fruit leaves was evaluated by determining the minimum inhibitory concentration (MIC) using broth dilution method. Results showed that the tropical fruit leaves extract exerted varying degrees of antibacterial activity towards the test organisms. Among these extracts, cold water extract of avocado and ethanol extracts of mango and star apple were the most effective against Escherichia coli. The hot water extract of mango, ethanol extract of star apple, and cold-water extract of sugar apple produced the highest zone of inhibition against Pseudomonas aeruginosa. Ethanol extract of avocado, hot water extract of guyabano and mango are the most effective against klebsiella pneumoniae. The minimum inhibitory concentration (MIC) of the tropical fruit leaf extracts were from 3.13 to 100 mg/ml. Ethanol proved to be the most effective solvent. <br/> 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Microbial sensitivity tests<br/>Antibiotics--Ananlysis<br/>Anti bacterial agents--Pharmacology.
<br/><br/><label>Dewey Class. No.: </label>615.329  / As9 2013