Antimicrobial activities of actinomycete isolates from soil samples in selected towns of upland Cavite against Escherichia coli O157, Staphylococcus aureus and Salmonella enterica from clinical isolates /
by Neil Edward C. Lubis.
- Indang, Cavite : Cavite State University- Main Campus, 2017.
- xiii, 61 pages : illustrations ; 28 cm.
Thesis (Doctor of Veterinary Medicine) Cavite State University
Includes bibliographical references.
College of Veterinary Medicine and Biomedical Sciences (VETMET) College of Veterinary Medicine and Biomedical Sciences (VETMET)
LUBIS, NEIL EDWARD C. Antimicrobial Activities of Actinomycete Isolates from Soil Samples in Selected Towns in Upland Cavite Against Escherichia coli O157, Staphylococcus aureus and Salmonella enterica from Clinical Isolates. Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. May 2017. Adviser: Dr. Nelson J. Montialto. The study was designed to isolate and screen antibiotic producing actinomycetes on samples obtained from soil from different towns in upland Cavite. A total of nineteen presumptive isolates from 12 soil samples were gathered. Actinomycete isolates were subjected to antimicrobial screening using agar plug technique against three different test bacteria. Evaluation of antimicrobial activity of isolates revealed that out of the 19 presumptive actinomycetes isolated, antimicrobial activity was observed in 9 (47.4%) isolates against the test organisms. Five (55%) of the isolates, showed the broadest spectrum of activity by inhibiting all of the test organisms; one (11.11%) isolate inhibited two of the test microorganisms and three (33.33%) isolates inhibited just one of the test organisms. Actinomycete isolates were characterized by cultural, morphological and biochemical means. The actinomycete isolates with antimicrobial activities were found to be opaque, with colonies ranging from 2 mm to 12 mm in size. The isolates came in different forms, mostly were circular while some were irregular. Adult colonies varied in color from having either brown, cream, yellow or white colonies. In terms of elevation, colonies varied from convex to crateriform, umbonate or undulate. Since many isolates showed antibacterial properties against test bacteria, it is suggestive that soils in upland Cavite could be an interesting source to explore for the discovery of novel actinomycetes and their antibacterial secondary metabolites. Further studies about the antibacterial properties of the actinomycete isolated in this study is encouraged. Actinomycete isolates that showed to possess antibacterial properties should be cultivated, their extract be collected and be used for a more detailed study of their antibacterial activity