Nuestro, Jennifer A.

An Evaluation of several media for use in identification of some fusarium species / Jennifer A. Nuestro. - Indang, Cavite : Cavite State University- Main Campus, 1999. - xi, 35 pages : illustrations ; 28 cm.

Thesis (BS Biology - - Microbiology) Cavite State University.

Includes bibliographical references.

College of Arts and Science (CAS) College of Arts and Science (CAS)

NUESTRO JENIFFER April 1999. "An Evaluation of Several Media for Use in Identification of Some Fusarium Species." An Undergraduate Thesis, Bachelor of Science in Biology, (Major in Microbiology). Thesis Adviser: Dr. AdelaidaE. Sangalang
Carnation leaf agar (CLA), cogon leaf agar (CGLA), banana leaf agar (BLA) and com leaf agar (CNLA) were compared for their usefulness in the identification of some Fusarium species, namely: F. solani, F. oxysporum, F. equiseti, Fusarium chlamydosporum, F moniliforme and F. proliferatum. The different media were evaluated based on the shape of macroconidia, the presence and formation of microconidia, production of sporodochia and chlamydospore, and cultural degeneration.
All cultures examined produced typical macroconidia and microconidia on CLA and CNLA. Chlamydospores were also produced consistently, and cultural degeneration was not observed. Thus, CLA and CNLA were the most suitable media for the identification of Fusar:um species used in this study. Conidial production was not consistent in BLA and CGLA and therefore, not suitable for the reliable identification of species examined.
Corn leaf agar is highly recommended as a substitute for CLA in the identification of Fusarium species.



Microbiology

576 / N88j 1999