Antimicrobial potential of fractions isolated from some selected leaves /
by Argel Joseph M. Mojica and Michael V. Pereņa.
- Indang, Cavite : Cavite State University- Main Campus, 2004.
- xiii, 66p. : ill. ; 28 cm. illustrations ; cm.
Thesis (BS Chemistry) Cavite State University.
Includes bibliographical references.
College of Arts and Science (CAS) College of Arts and Science (CAS)
MOJICA, ARGEL JOSEPH M., PERENA, MICHEL V. "Antimicrobial Potential of Fractions Isolated from Some Selected Leaves" An Undergraduate Thesis, B.S. Chemistry. Cavite State University, Indang, Cavite. April 2004. Adviser: Mrs. Dinah L. Espineli The inhibitory effect of plant extracts derived from three local medicinal herbs, namely, takip kuhol (Centella asiatica L.), damong maria (Artemesia vulgaris L.), and pansit-pansitan (Peperomia pellucida L.) on the growth of Staphylococcus aureus and Pseudomonas aeruginosa was studied. The polar and nonpolar fractions from the methanolic extract of air-dried leaves of the three plants were also screened for various phytochemicals. The most active fractions from each of the three samples based on antimicrobial and phytochemical results were subjected to infrared analysis. Results of phytochemical tests revealed the presence of flavonoids and saponins in all samples. Condensed tannins and cardiac glycosides were also present in certain fractions while none contained alkaloids and anthraquinone. Antimicrobial analysis led to the confirmation of the medicinal properties of these plants. Growth of S. aureus was greatly inhibited by the polar fractions of takip kuhol followed by damong maria and pansit-pansitan. On the contrary, the growth of P. aeruginosa was not inhibited by any of the fractions. It was further noted that nonpolar fractions had very little or no effect at all on the growth of both test organisms. Infrared analysis gave absorption peaks near 3400 cm-I for all fractions signifying the presence of OH groups, common for tannins and flavonoids. From these results, it was concluded that these phytochemicals could be accounted for the samples' antimicrobial activity.