Phytochemical analysis and evaluation of cytotoxicity and genotoxicity of breynia androgyna L. (Binahian) leaf extract /
by Christian Joshua S. Costa.
- Indang, Cavite : Cavite State University- Main Campus, 2019.
- xiv, 87 pages : illustrations ; 28 cm.
Thesis (Bachelor of Science in Biology Major in General Biology) Cavite State University.
Includes bibliographical references.
College of Arts and Sciences (CAS), Department of Biological Sciences College of Arts and Sciences (CAS), Department of Biological Sciences
COSTA, CHRISTIAN JOSHUA S. Phytochemical Analysis and Evaluation of Cytotoxicity and Genotoxicity of Breynia androgyna L. (Binahian) Leaf Extract. Undergraduate Thesis. Bachelor of Science in Biology major in General Biology. Cavite State University, Indang, Cavite. June 2019. Adviser: Ms. Liwayway P. Taglinao.
A study was conducted to assess the cytotoxicity and genotoxicity of Breynia androgyna L. (Binahian) ethanolic leaf extract using Allium cepa L. (Onion) test. Phytochemical analysis was performed on the leaf ethanolic extract of B. androgyna plant. In accordance, B. androgyna extract was diluted into different concentrations of plant extract as the treatment groups together with control groups. Six groups of onion bulbs were submerged into different treatment groups for two days and the morphological effects of the treatment were observed and documented. Onion roots were harvested and fixed for microscopic observation of the mitotic index and chromosomal aberrations.
This study showed that the phytochemical compounds that were present on the B. adrogyna consist of tannins, flavonoids, phenolics, alkaloids and steroids. There is a significant difference on the onion root length when submerged to B. androgyna extract in comparison with the negative control group (p<0.05). Thus, there is a significant inhibition of mitotic activity in the onion group treated with plant extract compared to the negative control (p<0.05). On the other hand, the inhibition of the mitotic activity was highly significant in the 750 ug/mL and 1000 gg/mL B. androgyna plant extract. One thousand gg/mL B. androgyna plant extract decreases mitotic index at 32% thus signifying it as having sublethal effects on an organism. The number of chromosomal aberrations increases as the level of concentration of plant extract increases compared to the negative control (tapwater). There was statistically significant differences at all the values except for 250 pg/ml B. androgyna extract concentration and 500 gg/mL B.androgyna extract concentration (p<0.05).Hence, the leaves of B. androgyna are commonly utilized by human as food or medicine as revealed by the survey. However, B. androgyna plant leaves, if consumed or applied at certain doses (approximately beyond 500 gg/mL), will induce a cytotoxic and genotoxic effect on an organism