Molecular detection of tick-borne flaviviruses in blood of Cattle in Cavite, Philippines / by Cherrymay O. Samaniego.

By:

Samaniego, Cherrymay O [author]

Contributor(s):

Talactac, Melbourne R [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2019.Description: xiv, 47 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

595.4 Sa4 2019

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Veterinary Medicine and Biological Science (CVMBS), Department of Clinical and Population Health

Abstract: SAMANIEGO, CHERRYMAY O. MOLECULAR DETECTION OF TICK- BORNE FLAVIVIRUSES IN BLOOD OF CATTLE IN CAVITE, PHILIPPINES. Thesis, Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. 2019. Adviser: Melbourne R. Talactac, DVM, MS, PhD. This study was conducted to detect the presence of tick- borne Flaviviruses (TBFV) in cattle from selected farms in Cavite. A total of one hundred blood samples were collected from the dorsal ear vein of cattle from different municipalities in Cavite. RNA samples extracted from the blood were used for complementary DNA (cDNA) synthesis. The cDNAs were initially used for the detection of mammalian Actin gene and later on, for the NS5 gene coding region conserved in TBFV using conventional Polymerase Chain Reaction (PCR). All blood samples were positive for mammalian Actin gene, however, TBFV were not detected. The result of this study suggested that TBFV may not be present in the areas where the samples were collected. Occurence of previous infection is possible but as a limitation of the study, PCR only detects present viremia in an animal through amplification of the target gene. The author recommends that further studies be conducted with larger sample size. Moreover, the time of collection should cover the warmer season because the presence of ticks in the area is important in spreading the virus. Lastly, animals should have tick infestation to be included in the study and serological tests should be utilized for improved detection.

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Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	595.4 Sa4 2019 (Browse shelf(Opens below))		Link to resource	Room use only	T-8113		00077367

Thesis (Doctor of Veterinary Medicine) Cavite State University.

Includes bibliographical references.

College of Veterinary Medicine and Biological Science (CVMBS), Department of Clinical and Population Health

SAMANIEGO, CHERRYMAY O. MOLECULAR DETECTION OF TICK- BORNE FLAVIVIRUSES IN BLOOD OF CATTLE IN CAVITE, PHILIPPINES. Thesis, Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. 2019. Adviser: Melbourne R. Talactac, DVM, MS, PhD.
This study was conducted to detect the presence of tick- borne Flaviviruses (TBFV) in cattle from selected farms in Cavite. A total of one hundred blood samples were collected from the dorsal ear vein of cattle from different municipalities in Cavite. RNA samples extracted from the blood were used for complementary DNA (cDNA) synthesis. The cDNAs were initially used for the detection of mammalian Actin gene and later on, for the NS5 gene coding region conserved in TBFV using conventional Polymerase Chain Reaction (PCR).
All blood samples were positive for mammalian Actin gene, however, TBFV were not detected. The result of this study suggested that TBFV may not be present in the areas where the samples were collected. Occurence of previous infection is possible but as a limitation of the study, PCR only detects present viremia in an animal through amplification of the target gene.
The author recommends that further studies be conducted with larger sample size. Moreover, the time of collection should cover the warmer season because the presence of ticks in the area is important in spreading the virus. Lastly, animals should have tick infestation to be included in the study and serological tests should be utilized for improved detection.

Submitted to the University Library June 24, 2019 T-8113

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