Reproductive biology and gestation of the male seahorse, Hippocampus barbouri (Jordan and Richardson 1908) / by Edna Perez Oconer.

By: Material type: TextTextLanguage: English Publication details: Quezon City : University of the Philippines, 2002.Description: xix, 131 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 591.77 Oc5 2002
Online resources: Abstract: Seahorse, Hippocampus barbouri (Jordan-Richardson 1908) is one of the endangered species worldwide. Attempts to breed the fish in captivity are hampered by many unanswered questions regarding its general reproductive biology, especially its gestation. This study is the first investigation of the gross morphology of reproductive system, histology and ultrastructure of the brood pouch, and localization of sex steroids through immunohistochemistry and enzyme histochemistry. Results showed that seahorse reproductive system does not differ from a typical male teleost except for the presence of brooding pouch in males and was evident starting at 8 cm length. The seahorse testis does not form spermatocyst and belongs to unrestricted spermatogonial type. Onset of sex differentiation was observed at size length 4 to 6 cm. Ultrastructural study revealed the absence of structural connection between the embryo and the capsules formed by the pouch wall. Immunohistochemistry, enzyme histochemistry and chemiluminescence studies showed synthesis of the steroids, estrogen and progesterone in the testes, brood pouch and interrenal gland and their possible involvement in gestation by their abundance during gestation. These findings will be valuable in the aquaculture and conservation of this species.
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Item type Current library Collection Call number Materials specified URL Copy number Status Notes Date due Barcode
Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 591.77 Oc5 2002 (Browse shelf(Opens below)) Link to resource c1 Room use only T-5944 00000040
Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 591.77 Oc5 2002 (Browse shelf(Opens below)) Link to resource c2 Room use only T-5976 00000041

Thesis (Ph.D. Biology) University of the Philippines

Includes bibliographical references.

Seahorse, Hippocampus barbouri (Jordan-Richardson 1908) is one of the endangered species worldwide. Attempts to breed the fish in captivity are hampered by many unanswered questions regarding its general reproductive biology, especially its gestation. This study is the first investigation of the gross morphology of reproductive system, histology and ultrastructure of the brood pouch, and localization of sex steroids through immunohistochemistry and enzyme histochemistry.

Results showed that seahorse reproductive system does not differ from a typical male teleost except for the presence of brooding pouch in males and was evident starting at 8 cm length. The seahorse testis does not form spermatocyst and belongs to unrestricted spermatogonial type.

Onset of sex differentiation was observed at size length 4 to 6 cm. Ultrastructural study revealed the absence of structural connection between the embryo and the capsules formed by the pouch wall.

Immunohistochemistry, enzyme histochemistry and chemiluminescence studies showed synthesis of the steroids, estrogen and progesterone in the testes, brood pouch and interrenal gland and their possible involvement in gestation by their abundance during gestation. These findings will be valuable in the aquaculture and conservation of this species.


Submitted to the University Library 5/27/2016 T-5944 c1

Submitted to the University Library 5/27/2016 T-5976 c2

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