Antimicrobial and antihelmintic activities of actinomycetes from soil samples in selected upland towns of Cavite / by Nelson Javier Montialto.

By:

Montialto, Nelson Javier [author]

Contributor(s):

Ilagan, Yolanda A [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2014. Cavite State University- Main Campus,Description: xv, 92 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

579 M76 2014

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

Graduate Studies Open Learning College (GSOLC)

Abstract: MONTIALTO, NELSON J., April 2014. Antimicrobial and Anthelmintic Activities of Actinomycetes from Soil Samples in Selected Upland Towns of Cavite. Graduate Thesis. Master of Science in Biology. Cavite State University, Indang, Cavite. Adviser: Dr. Yolanda A. Ilagan. A study was conducted to screen for actinomycetes with potential bioactive properties. A total of 50 soil samples from five municipalities in upland Cavite were obtained for the isolation and screening of actinomycetes with antimicrobial and anthelminthic activities. Actinomycete isolates were subjected to antimicrobial screening by agar plug and liquid assays while culture supernatant assay was done to determine anthelminthic activity of the isolates. All positive isolates were characterized morphologically and biochemically. Among the 32 presumptive actinomycetes isolated, 17 (53.12%) isolates showed antimicrobial activities. Five (15.62%) of these isolates showed the broadest spectrum of activity inhibiting all five of the test organisms; five (15.62%) isolates inhibited four of the test organisms; five (15.62%) isolates inhibited three of the test organisms and only two (6.25%) isolates inhibited just two of the test organisms. Likewise, the results of the anthelminthics assay revealed that nine isolates possessed anthelminthic activity, capable of inhibiting the development of less than 50% of the tested parasitic ova. Positive isolates were able to produce small to large-sized colonies ranging from 1-9 mm, chalky to powdery and velvety texture, irregular to regular, flat to raised colonies, compact and somewhat embedded in the medium. With regards to the color of aerial mycelium, the isolates can be grouped as white, gray, yellow and red Microscopically, three categories were recognized in this study: straight to flexuous (rectiflexibles), hooks, loops, or spirals with turns (retinaculiaperti) and spiral (spirals). Based on morphological and biochemical characterization, all isolates were tentatively classified as species belonging to the genus Streptomyces. Considering the outcome of the present investigation, the soils of upland Cavite are good candidates for deriving economically important actinomycetes. Thus, there is a definite scope for bioprospecting of antagonistic actinomycetes in the soils of Cavite.

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Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	579 M76 2014 (Browse shelf(Opens below))		Link to resource	Room use only	T-6481		00010262

Thesis (Master of Science in Biology) Cavite State University

Includes bibliographical references.

Graduate Studies Open Learning College (GSOLC)

MONTIALTO, NELSON J., April 2014. Antimicrobial and Anthelmintic Activities of Actinomycetes from Soil Samples in Selected Upland Towns of Cavite.
Graduate Thesis. Master of Science in Biology. Cavite State University, Indang, Cavite. Adviser: Dr. Yolanda A. Ilagan.
A study was conducted to screen for actinomycetes with potential bioactive properties. A total of 50 soil samples from five municipalities in upland Cavite were obtained for the isolation and screening of actinomycetes with antimicrobial and anthelminthic activities. Actinomycete isolates were subjected to antimicrobial screening by agar plug and liquid assays while culture supernatant assay was done to determine anthelminthic activity of the isolates. All positive isolates were characterized morphologically and biochemically.
Among the 32 presumptive actinomycetes isolated, 17 (53.12%) isolates showed antimicrobial activities. Five (15.62%) of these isolates showed the broadest spectrum of activity inhibiting all five of the test organisms; five (15.62%) isolates inhibited four of the test organisms; five (15.62%) isolates inhibited three of the test organisms and only two (6.25%) isolates inhibited just two of the test organisms. Likewise, the results of the anthelminthics assay revealed that nine isolates possessed anthelminthic activity, capable of inhibiting the development of less than 50% of the tested parasitic ova.
Positive isolates were able to produce small to large-sized colonies ranging from 1-9 mm, chalky to powdery and velvety texture, irregular to regular, flat to raised colonies, compact and somewhat embedded in the medium. With regards to the color of aerial mycelium, the isolates can be grouped as white, gray, yellow and red Microscopically, three categories were recognized in this study: straight to flexuous (rectiflexibles), hooks, loops, or spirals with turns (retinaculiaperti) and spiral (spirals). Based on morphological and biochemical characterization, all isolates were tentatively classified as species belonging to the genus Streptomyces. Considering the outcome of the present investigation, the soils of upland Cavite are good candidates for deriving economically important actinomycetes. Thus, there is a definite scope for bioprospecting of antagonistic actinomycetes in the soils of Cavite.

Submitted copy to the University Library. 02/14/2017 T-6481

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