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Antibiofilm and cytotoxic activity of pungapung (Amorphophallus campanulatus Roxb.) extracts / by Dhenicka Vivien C. Villanueva.

By:

Villanueva, Dhenicka Vivien C [author]

Contributor(s):

Ilagan, Yolanda A [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2019.Description: xv, 125 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

616.904 V71 2019

Online resources:

Click here to view abstract and table of contents

Production credits:

College of Arts and Sciences (CAS), Department of Biological Sciences

Abstract: VILLANUEVA, DHENICKA VIVIEN CABREROS. ANTIBIOFILM AND CYTOTOXIC ACTIVITY OF PUNGAPUNG (Amorphophallus campanulatus Roxb.) EXTRACTS. Undergraduate Thesis, Bachelor of Science in Biology. Cavite State University, Indang, Cavite. June 2019. Adviser: Dr. Yolanda A. Ilagan. A study was conducted to assess the antibacterial, antibiofilm, and cytotoxic activities of pungapung (Amorphophallus campanulatus Roxb.) against Escherichia coli B1825, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 12600. Leaves and stems of A. campanulatus were subjected to different extraction procedures: decoction, crude juice, dichloromethane, and methanolic extracts and were tested against these test organisms. Disk-diffusion assay showed that only decoction and methanolic extracts exhibited antibacterial activity against E. coli. Minimum inhibitory concentration (MIC) of these extracts were determined using resazurin-based microtiter plate assay. The MIC for decoction and methanolic extracts were observed at 50 mg/ml and 40 mg/ml against E. coli. The extracts were then tested for antibiofilm activity using biofilm inhibitory assay in different wavelengths (450 nm, 490 nm, and 630 nm). Analysis of Variance (ANOVA) revealed that decoction and methanolic extracts were better than gentamicin in inhibitory biofilm formation. Cytotoxic activity of the extracts was determined using brine shrimp lethality assay (Artemia salina) using various concentrations. Results revealed that the highest death percentage (95.56%) was caused by methanolic stem extract followed by methanolic leaf extract (86.67%) at 1000 ppm concentration. The estimated LCso value based on probit analysis after six hours was 10.09 ppm. Decoction extracts were considered less toxic than methanolic extracts.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	616.904 V71 2019 (Browse shelf(Opens below))		Link to resource	Room use only	T-8344		00079650

Thesis (Bachelor of Science in Biology) Cavite State University.

Includes bibliographical references.

College of Arts and Sciences (CAS), Department of Biological Sciences

VILLANUEVA, DHENICKA VIVIEN CABREROS. ANTIBIOFILM AND CYTOTOXIC ACTIVITY OF PUNGAPUNG (Amorphophallus campanulatus Roxb.) EXTRACTS. Undergraduate Thesis, Bachelor of Science in Biology. Cavite State University, Indang, Cavite. June 2019. Adviser: Dr. Yolanda A. Ilagan.

A study was conducted to assess the antibacterial, antibiofilm, and cytotoxic activities of pungapung (Amorphophallus campanulatus Roxb.) against Escherichia coli B1825, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 12600.

Leaves and stems of A. campanulatus were subjected to different extraction procedures: decoction, crude juice, dichloromethane, and methanolic extracts and were tested against these test organisms. Disk-diffusion assay showed that only decoction and methanolic extracts exhibited antibacterial activity against E. coli. Minimum inhibitory concentration (MIC) of these extracts were determined using resazurin-based microtiter plate assay. The MIC for decoction and methanolic extracts were observed at 50 mg/ml and 40 mg/ml against E. coli. The extracts were then tested for antibiofilm activity using biofilm inhibitory assay in different wavelengths (450 nm, 490 nm, and 630 nm). Analysis of Variance (ANOVA) revealed that decoction and methanolic extracts were better than gentamicin in inhibitory biofilm formation. Cytotoxic activity of the extracts was determined using brine shrimp lethality assay (Artemia salina) using various concentrations. Results revealed that the highest death percentage (95.56%) was caused by methanolic stem extract followed by methanolic leaf extract (86.67%) at 1000 ppm concentration. The estimated LCso value based on probit analysis after six hours was 10.09 ppm. Decoction extracts were considered less toxic than methanolic extracts.

Submitted to the University Library 07/18/2019 T-8344