Bacterial endophytes as potential biological control agents of coffee rust / by Liwayway P. Taglinao.

By:

Taglinao, Liwayway P [author]

Contributor(s):

Ilagan, Yolanda A [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2016.Description: xvi, 117 pages : 28 cm. illustrationsContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

630.2 T12 2016

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Arts and Science (CAS)

Abstract: TAGLINAO, LIWAYWAY PERLADO, December 2016, "Bacterial Endophytes as Biological Control Agents of Coffee Rust". Master's Thesis, Master of Science in Biology, Cavite State University, Indang, Cavite: Adviser: Dr. Yolanda A. Ilagan This study was conducted to isolate and identify bacterial endophytes found in healthy and infected coffee leaves with potential to inhibit coffee rust. A total of 145 bacterial endophytes were isolated from five coffee-growing towns of Cavite, namely: Tagaytay„4madeo, Alfonso, Silarig and Indang. These bacterial endophytes were screened for their biological control potential against the yeasts, Candida utilis and Saccharomyces cerevisiae, and molds, Aspergillus niger and Fusarium oxysporum. Twenty nine of these endophytes were positive for inhibitory activity. Three endophytes were able to inhibit all the four test organisms, two inhibited only three, five were effective against two fungal organisms while 19 of the isolates were effective against only one test organism. These bacterial endophytes were used to test whether they can control rust in coffee leaf discs. One of the bacterial isolates (IAMA 1) did not produce lesion when applied 24 hours before the inoculation of the rust pathogen. The means of percentage lesions produced by H. vastatrix were comparable to the means of percentage lesions when leaf discs were treated with Dithane, thus this indicates that rust pathogen was totally controlled. Five bacterial endophytes (ILMI, IRMA 2, ALRMA, IAMAI, IRMA 1) when applied simultaneously with rust pathogen also inhibited the growth of H. vastairx comparable to the effect of Dithane. Based on morphological and physiological characteristics, the bacterial isolates positive for inhibition belonged into six genera including Citrobacter, Klebsiella, Enterobacter, Corynebacterium, Bacillus, and Serratia.

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Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	630.2 T12 2016 (Browse shelf(Opens below))		Link to resource	Room use only	T-6101		00009876

Thesis (MS Biology) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

TAGLINAO, LIWAYWAY PERLADO, December 2016, "Bacterial Endophytes as Biological Control Agents of Coffee Rust". Master's Thesis, Master of Science in Biology, Cavite State University, Indang, Cavite: Adviser: Dr. Yolanda A. Ilagan

This study was conducted to isolate and identify bacterial endophytes found in healthy and infected coffee leaves with potential to inhibit coffee rust. A total of 145 bacterial endophytes were isolated from five coffee-growing towns of Cavite, namely: Tagaytay„4madeo, Alfonso, Silarig and Indang. These bacterial endophytes were screened for their biological control potential against the yeasts, Candida utilis and Saccharomyces cerevisiae, and molds, Aspergillus niger and Fusarium oxysporum. Twenty nine of these endophytes were positive for inhibitory activity. Three endophytes were able to inhibit all the four test organisms, two inhibited only three, five were effective against two fungal organisms while 19 of the isolates were effective against only one test organism.

These bacterial endophytes were used to test whether they can control rust in coffee leaf discs. One of the bacterial isolates (IAMA 1) did not produce lesion when applied 24 hours before the inoculation of the rust pathogen. The means of percentage lesions produced by H. vastatrix were comparable to the means of percentage lesions when leaf discs were treated with Dithane, thus this indicates that rust pathogen was totally controlled. Five bacterial endophytes (ILMI, IRMA 2, ALRMA, IAMAI, IRMA 1) when applied simultaneously with rust pathogen also inhibited the growth of H. vastairx comparable to the effect of Dithane. Based on morphological and physiological characteristics, the bacterial isolates positive for inhibition belonged into six genera including Citrobacter, Klebsiella, Enterobacter, Corynebacterium, Bacillus, and Serratia.

Submitted copy to the University Library. 01/04/2017 T-6101

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