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Immune response of Nile Tilapia (Oreochromis niloticus) on Aeromonas hydrophila isolated from Taal Lake / by Jenielyn V. Poniente.

By:

Poniente, Jenielyn V [author]

Contributor(s):

Ilagan, Yolanda A [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2002. Cavite State University- Main Campus,Description: xii, 53 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

639.8 P77 2002

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Arts and Science (CAS)

Abstract: PONIENTE, JENIELYN VELASQUEZ, April, 2002, " Immune Response of tilapia (Oreochromis niloticus) on Aeromonas hydrophila Isolated from Taal Lake" An Undergraduate Thesis, Bachelor of Science in Biology, major in Microbiology, Cavite State University, Indang, Cavite. Advisers: Mr. Francisco M. Heralde Ill and Dr. Yolanda A. Ilagan Immune response of Nile tilapia (Oreochromis niloticus) on Aeromonas hydrohila isolated from Taal Lake was conducted. This study aimed to: optimize the conditions for tilapia blood sample extraction and processing, antibody quantification and white and red blood cells detection and quantification and to determine the effect of Aeromonas hydrophila infection on the tilapia antibody titers and levels of white and red blood cells, physical appearance and mortality rate. Four treatments were employed: sterile distilled water as control, heat-killed bacteria, formalin- killed bacteria and bacteria suspended in saline solution at a rate of 2.15 x 105 cells per ml. It was found out that 10ul of inoculum per gram offish can be used as a standard concentration of antigen injected to fish considering their body weight. Positive agglutination was observed in Nile tilapia after injection of Aeromonas hydrophila. Increase in antibody titer of fish at all treatments was detected on the third day of post- inoculation up to ten days. Rapid decline of antibody production was observed on the tenth day of post- inoculation and onwards. Red blood cell (RBC) of Nile tilapia at different treatments except for the control declines after six days post- inoculation Decrease in leukocyte count of fish given heatkilled, formalin-killed and live bacteria suspended in 0.85% saline solution was observed after stimulation of antigen. During the conduct of the study, all fish samples exhibited symptoms of disease caused by Aeromona hydrophila such as exopthalmia, fin rot and lost of scales.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	639.8 P77 2002 (Browse shelf(Opens below))		Link to resource	Room use only	T-2389		00006411

Thesis (B.S. Biology--Microbiology) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

PONIENTE, JENIELYN VELASQUEZ, April, 2002, " Immune Response of tilapia (Oreochromis niloticus) on Aeromonas hydrophila Isolated from Taal Lake" An Undergraduate Thesis, Bachelor of Science in Biology, major in Microbiology, Cavite State University, Indang, Cavite. Advisers: Mr. Francisco M. Heralde Ill and Dr. Yolanda A. Ilagan
Immune response of Nile tilapia (Oreochromis niloticus) on Aeromonas hydrohila isolated from Taal Lake was conducted. This study aimed to: optimize the conditions for tilapia blood sample extraction and processing, antibody quantification and white and red blood cells detection and quantification and to determine the effect of Aeromonas hydrophila infection on the tilapia antibody titers and levels of white and red blood cells, physical appearance and mortality rate. Four treatments were employed: sterile distilled water as control, heat-killed bacteria, formalin- killed bacteria and bacteria suspended in saline solution at a rate of 2.15 x 105 cells per ml.
It was found out that 10ul of inoculum per gram offish can be used as a standard concentration of antigen injected to fish considering their body weight.
Positive agglutination was observed in Nile tilapia after injection of Aeromonas hydrophila. Increase in antibody titer of fish at all treatments was detected on the third day of post- inoculation up to ten days. Rapid decline of antibody production was observed on the tenth day of post- inoculation and onwards.
Red blood cell (RBC) of Nile tilapia at different treatments except for the control declines after six days post- inoculation Decrease in leukocyte count of fish given heatkilled, formalin-killed and live bacteria suspended in 0.85% saline solution was observed
after stimulation of antigen.
During the conduct of the study, all fish samples exhibited symptoms of disease caused by Aeromona hydrophila such as exopthalmia, fin rot and lost of scales.

Submitted to the University Library 05/10/2007 T-2389