Antifungal activity of some medicinal plants and their synergistic effects with antibiotics on some species of candida causing candidiasis / by Florisa Mae A. Ilagan and Anna Patricia N. Ramos.
Material type:
TextLanguage: English Publication details: Indang, Cavite : 2010. Cavite State University- Main Campus,Description: xiv, 64 pages : 28 cm. illustrationsContent type: - text
- unmediated
- volume
- 616.969 Il1 2010
- Science High School
| Item type | Current library | Collection | Call number | Materials specified | URL | Status | Notes | Date due | Barcode |
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Theses / Manuscripts
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Ladislao N. Diwa Memorial Library Theses Section | Non-fiction | 616.969 Il1 2010 (Browse shelf(Opens below)) | Link to resource | Room use only | RS-585 | 00007917 |
Research Study (Science High School) Cavite State University
Includes bibliographical references.
Science High School
ILAGAN, IFLORIESA MAE A. and RAMOS, ANNA PATRICIA N. Anlifungall Activity of Some Medicinal Plants and their Synergistic Effects with Antibiotics on Some Species of Candida causing Candidiasis. Research Study (General Science Curiculum). Science High School, College of Education, Cavite State University. Indang, Cavite. March 2010. Adviser: Dr. Yolanda A. Ilagan
This study was conducted at Cavite State University, Indang, Cavite from June to November 2009. Generally, this study aimed to evaluate the in vitro antifungal activity of selected medicinal plant extracts against clinical isolates of Candida species. Specifically, it aimed to identify which among the selected medicinal plants are inhibitory to some Candida species; determine the phytochemicals present in various medicinal plant extracts with antifungal activity; and analyze if there is synergistic or antagonistic reactions between the antifungal antibiotics and plant extracts. Thirty (30) plant samples were used in the preparation of dichloromethane, methanol, decoction and crude extracts. These extracts were tested against three species of Candida (C albicans, C parapsilosis, and C krusei) using the disk diffusion assay.
Among the dichloromethane extracts of medicinal plants, five were able to inhibit C. albicans; 10 inhibited C. parapsilosis; and 23 showed antifungal activity against C. krusei. Results also showed that the dichloromethane extract of calamansi leaves (Citrus microcorpa Bunge) displayed excellent inhibitory activity against the Candida species. Likewise, the decoction and crude extract of garlic (Allium sativum) displayed positive zone of inhibition against C. species. On the other hand, the methanolic extract of madre de cacao (Gliridia sepium Steud.) was observed to inhibit C. albicans only. Qualitative phytochemical analyses revealed the presence of various compounds in the extract. Crude juice of garlic contained tannins, resin, oil and carbohydrates. Garlic decoction had resin, oil, saponin and flavonoids. Saponin, flavonoids and carbohydrates were present in methanolic extract of madre de cacao.
Likewise, dichloromethane extract of calamansi contained tannins, resin, saponin, and flavonoids. Other plant samples inhibitory to Candida species had at least one pythochemical present. For C. albicans, garlic juice when combined with amphotericin B exhibited either antagonism or additive effect depending on the volume of amphotericin B in which they were compared with. Garlic decoction and methanolic extract of madre de cacao showed antagonistic effect when used in combination with amphotericin B. Synergism and additive effects, however, were observed among the majority of DCM extracts. For C. krusei, garlic juice and decoctions showed either additive, antagonistic or synergistic effects when used with amphotericin B while basil gave synergistic effects in two concentrations tested.
Submitted to the University Library 05/04/2010 RS-585