Isolation, identification and biological control activity of endophytic bacteria associated with Coffee leaves / by Erica Jean S. Villanueva.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite, 2013. Cavite State University - Main CampusDescription: xii, 70 pages : 28 cm. illustrationsContent type:
  • text
Media type:
  • unmedaited
Carrier type:
  • volume
Subject(s): DDC classification:
  • 589.9  V71 2013
Online resources: Production credits:
  • College of Arts and Science (CAS)
Abstract: VILLANUEVA, ERICKA JEAN SACALA, March 2013, "Isolation, Identification and Biological Control Activity of Endophytic Bacteria Associated with Coffee Leaves". Undergraduate Thesis, Bachelor of Science in Biology, Major in General Biology, Cavite State University, Indang, Cavite. Adviser: Dr. Yolanda A. Ilagan This study was done to isolate and identify bacterial endophytes present in four varieties of coffee. Specifically, this aimed to differentiate the bacterial endophytes from four varieties of coffee based on their morphological and physiological characteristics; and determine which among these bacterial endophytes can serve as effective biocontrol agents against Ralstonia solanacearum, Aspergillus niger, and Erwinia carotovora. Fifty-seven isolates were obtained from coffee leaves collected in Amadeo (n = 20), Alfonso (n = 17), and Indang (n = 20). Based on morphological, cultural and physiological tests, the isolates were identified as Enterobacter aerogenes and Enterobacter cloacae, Serratia marsecens, Bacillus sp, Alcaligenes sp, and Aminobacter sp. These bacteria are found in coffee regardless of town and varieties sampled. Four out of five genera exhibited an inhibitory effect against A. niger but none to R. solanacearum and E. carotovora.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 589.9 V71 2013 (Browse shelf(Opens below)) Room use only T-5224 00008371

Thesis ( BS Biology ) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

VILLANUEVA, ERICKA JEAN SACALA, March 2013, "Isolation, Identification and Biological Control Activity of Endophytic Bacteria Associated with Coffee Leaves". Undergraduate Thesis, Bachelor of Science in Biology, Major in General Biology, Cavite State University, Indang, Cavite. Adviser: Dr. Yolanda A. Ilagan
This study was done to isolate and identify bacterial endophytes present in four varieties of coffee. Specifically, this aimed to differentiate the bacterial endophytes from four varieties of coffee based on their morphological and physiological characteristics; and determine which among these bacterial endophytes can serve as effective biocontrol agents against Ralstonia solanacearum, Aspergillus niger, and Erwinia carotovora. Fifty-seven isolates were obtained from coffee leaves collected in Amadeo (n = 20), Alfonso (n = 17), and Indang (n = 20). Based on morphological, cultural and physiological tests, the isolates were identified as Enterobacter aerogenes and Enterobacter cloacae, Serratia marsecens, Bacillus sp, Alcaligenes sp, and Aminobacter sp. These bacteria are found in coffee regardless of town and varieties sampled. Four out of five genera exhibited an inhibitory effect against A. niger but none to R. solanacearum and E. carotovora.

Submitted to the University Library 07/31/2013 T-5224

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