Clinico-mycological profiles of superficial fungal pathogens isolated from skin and nails of infected individuals residing in Naic, Cavite / by Tricia Emily C. Tablan.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2015. Cavite State University- Main Campus,Description: xvi, 135 pages : 28 cm. illustrations ; Content type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 579.5  T11 2015
Online resources: Production credits:
  • College of Arts and Science (CAS)
Abstract: TABLAN, TRICIA EMILY C. “CLINICO-MYCOLOGICAL PROFILES OF SUPERFICIAL FUNGAL PATHOGENS ISOLATED FROM SKIN AND NAILS. Thesis, Bachelor of Science in Biology, Major in Microbiology. College of Arts and Sciences, Cavite State University, Indang, Cavite. 2015. Adviser: Prof. Sherine M. Cruzate. Superficial fungal infections such as dermatophytosis, pityriasis versicolor and non-dermatophyte fungal infections are extremely common on tropical countries like the Philippines and the warm and humid conditions favours the growth of pathogenic fungi. Forty five infected individuals residing in three barangays of Naic namely: Bucana Sasahan, Timalan Balsahan, and Malainen Bago exhibited symptoms of superficial mycoses. The most common infections were tinea unguium, tinea corporis, and tinea pedis. Several individuals also showed multiple infections in their body. Most infected individuals were female belonging to age groups <10 years old and 51 — 60. With regard to living conditions and hygienic practices infected individuals take their bath at least once a day. However, 29 (64.44%) of the affected individuals do not use their own bath towel while 17 (37.78%) used their dirty clothes to wipe their body. Thirteen (28.89%) individuals experienced overcrowding in their house having more than seven family members. A total of 169 clinical specimens, comprised of skin scrapings, nail clippings and skin swabs were collected. Direct microscopic examination (KOH m unt) showed that all samples were positive with the presence of fungal hyphae and spores. However, not all KOH positive samples were culture positive. Cultural and morphological characterization of the fungal isolates revealed that aside from dermatophyte Microsporum audouinii, non-dermatophyte fungi were associated with superficial fungal infections. These were identified belonging to genera Aspergillus, | Acremonium, . Mucor, Penicillium, Scopulariopsis, Paecilomyces, Colletotrichum, Scedosporium, Rhizomucor, Rhizopus oryzae, and Chaetomium. Among these, five species of Aspergillus such as 4. flavus, A. fumigatus, A. nidulans, A. niger and A. terreus and two species of Penicillium such as Penicillium sp. and Penicillium cheresanum were commonly observed on individuals suffering with tinea unguium and tinea corporis. Statistical analysis revealed that age, gender, nature of work, and hygienic practices of the participants were significantly related to the occurrence of fungal pathogens. Likewise, tinea unguium, tinea pedis, tinea manuum, and tinea faciale are all significantly related to the superficial fungal pathogens obtained from the infected individuals.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 579.5 T11 2015 (Browse shelf(Opens below)) Link to resource Room use only T-6168 00010139

Thesis (Bachelor of Science in Biology) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

TABLAN, TRICIA EMILY C. “CLINICO-MYCOLOGICAL PROFILES OF SUPERFICIAL FUNGAL PATHOGENS ISOLATED FROM SKIN AND NAILS. Thesis, Bachelor of Science in Biology, Major in Microbiology. College of Arts and Sciences, Cavite State University, Indang, Cavite. 2015. Adviser: Prof. Sherine M. Cruzate.

Superficial fungal infections such as dermatophytosis, pityriasis versicolor and non-dermatophyte fungal infections are extremely common on tropical countries like the Philippines and the warm and humid conditions favours the growth of pathogenic fungi.

Forty five infected individuals residing in three barangays of Naic namely: Bucana Sasahan, Timalan Balsahan, and Malainen Bago exhibited symptoms of superficial mycoses. The most common infections were tinea unguium, tinea corporis, and tinea pedis. Several individuals also showed multiple infections in their body. Most infected individuals were female belonging to age groups <10 years old and 51 — 60. With regard to living conditions and hygienic practices infected individuals take their bath at least once a day. However, 29 (64.44%) of the affected individuals do not use their own bath towel while 17 (37.78%) used their dirty clothes to wipe their body.

Thirteen (28.89%) individuals experienced overcrowding in their house having more than seven family members.

A total of 169 clinical specimens, comprised of skin scrapings, nail clippings and skin swabs were collected. Direct microscopic examination (KOH m unt) showed that all samples were positive with the presence of fungal hyphae and spores. However, not all KOH positive samples were culture positive.

Cultural and morphological characterization of the fungal isolates revealed that aside from dermatophyte Microsporum audouinii, non-dermatophyte fungi were associated with superficial fungal infections. These were identified belonging to genera Aspergillus, | Acremonium, . Mucor, Penicillium, Scopulariopsis, Paecilomyces, Colletotrichum, Scedosporium, Rhizomucor, Rhizopus oryzae, and Chaetomium. Among these, five species of Aspergillus such as 4. flavus, A. fumigatus, A. nidulans, A. niger and A. terreus and two species of Penicillium such as Penicillium sp. and Penicillium cheresanum were commonly observed on individuals suffering with tinea unguium and tinea corporis.

Statistical analysis revealed that age, gender, nature of work, and hygienic practices of the participants were significantly related to the occurrence of fungal pathogens.
Likewise, tinea unguium, tinea pedis, tinea manuum, and tinea faciale are all significantly related to the superficial fungal pathogens obtained from the infected individuals.

Submitted copy to the University Library. 02/14/2017 T-6168

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