Screening of chitinolytic actinomycetes as potential biological control agent against agricultural pests / by Maria Elielou R. Notario.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2017. Cavite State University- Main Campus,Description: xiv, 95 pages : 28 cm. illustrations ; Content type:
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  • unmediated
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  • volume
Subject(s): DDC classification:
  • 632  N84 2017
Online resources: Production credits:
  • College of Arts and Science (CAS)
Abstract: NOTARIO, MARIA ELIELOU R. Screening of Chitinolytic Actinomycetes as Potential Biological Control Agent Against Agricultural Pests. Undergraduate thesis. Bachelor of Science in Biology, Cavite State University, Indang Cavite, May 2017. Adviser: Prof. Sherine M. Cruzate. This study was conducted to isolate, identify and screen chitinolytic Actinomycetes from agricultural soil samples of Cavite, as biological control agent against fire ants (Selonopsis invicta), red weaver ants (Oecophylla sp.), and termites (Coptotermes sp.) based on mortality rate and to compare the efficiency of chitinolytic Actinomycetes as biological control agent with the commercially available insecticide. Out of 45 actinomycete isolated from 12 agricultural soil samples of Cavite, five actinomycete isolates (S3B2, SIA3, S6B2, S5B8, and S2A(A1)) exhibited high chitinase activity based on abundant growth and fast growth rate in Colloidal Chitin Agar (CCA) and Inorganic Salt Starch Agar (SSA). Cultural, morphological and biochemical characterization revealed that these isolates belong to four different species of Streptomyces and Saccharomonospora. Bioassay against fire ants, weaver ants and termites revealed that malathion was the most effective, killing all the test insects on the first four hours of incubation. However, prolonged exposure of chitinolytic actinomycetes to the test insects resulted to increase in mean mortality rate. Streptomyces species 2 was found to be effective against fire ants and red weaver, killing all the test insects after 36 hours of exposure. On the other hand, Streptomyces species 3 killed all the termites after 16 hours of exposure. Statistical analysis revealed that the mean mortality of malathion in all the test insects were significantly different with the mean monality of the chitinolytic actinomycetes in the first four hours of exposure. However, prolonged exposure of the test insects to actinomycetes resulted to mean mortality which was not significantly different with malathion. This implies that prolonged exposure of chitinolytic actinomycetes can kill the test insects comparable to commercial insecticide.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 632 N84 2017 (Browse shelf(Opens below)) Link to resource Room use only T-6870 00011238

Thesis (BS Biology) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

NOTARIO, MARIA ELIELOU R. Screening of Chitinolytic Actinomycetes as Potential Biological Control Agent Against Agricultural Pests. Undergraduate thesis. Bachelor of Science in Biology, Cavite State University, Indang Cavite, May 2017. Adviser: Prof. Sherine M. Cruzate.
This study was conducted to isolate, identify and screen chitinolytic Actinomycetes from agricultural soil samples of Cavite, as biological control agent against fire ants (Selonopsis invicta), red weaver ants (Oecophylla sp.), and termites (Coptotermes sp.) based on mortality rate and to compare the efficiency of chitinolytic Actinomycetes as biological control agent with the commercially available insecticide. Out of 45 actinomycete isolated from 12 agricultural soil samples of Cavite, five actinomycete isolates (S3B2, SIA3, S6B2, S5B8, and S2A(A1)) exhibited high chitinase activity based on abundant growth and fast growth rate in Colloidal Chitin Agar (CCA) and Inorganic Salt Starch Agar (SSA). Cultural, morphological and biochemical characterization revealed that these isolates belong to four different species of Streptomyces and Saccharomonospora. Bioassay against fire ants, weaver ants and termites revealed that malathion was the most effective, killing all the test insects on the first four hours of incubation. However, prolonged exposure of chitinolytic actinomycetes to the test insects resulted to increase in mean mortality rate. Streptomyces species 2 was found to be effective against fire ants and red weaver, killing all the test insects after 36 hours of exposure. On the other hand, Streptomyces species 3 killed all the termites after
16 hours of exposure. Statistical analysis revealed that the mean mortality of malathion in all the test insects were significantly different with the mean monality of the chitinolytic actinomycetes in the first four hours of exposure. However, prolonged exposure of the test insects to actinomycetes resulted to mean mortality which was not significantly different with malathion. This implies that prolonged exposure of chitinolytic actinomycetes can kill the test insects comparable to commercial insecticide.

Submitted copy to the University Library. 08/03/2017 T-6870

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