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008			070511s19uu xx 00 eng d
040			_cCvSU Main Campus Library
041	0		_aeng
082	0	4	_a581.8 _bP94 1981
100		0	_aPromtep, Kongsakdi _eauthor _942185
245	1	0	_aEmbryo and tissue culture of Strelitzia reginae Ait. / _cKongsakdi Promtep.
260	0		_aLos Baños, Laguna : _bUniversity of the Philippines- Los Baños, _c1981.
300			_a196 pages : _billustrations ; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
500			_aThesis (Ph.D. - - Horticulture) University of the Philippines, College, Laguna.
504			_aIncludes bibliographical references.
520	3		_a KONGSAKDI PROMTEP, University of the Philippines at Los Banos, July 1981. Embryo and Tissue Culture of Strelitzia Reginae Ait. Major Professor: Dr. Helen L. Valmeyor,. Two sets of experiments were conducted to explore the potential of in vitro method for propagating the excised embryo and shoot tip of Strelitzia reginae. The excised embryo germinate readily in Whitets medium without vitamins in 7 days. Compared to other basal media tested, highly modified Murashige and Skoog's medium (MS) enhanced the growth of seedlings. With MS bosal media, the growth of seedlings from embryos wes accelerated when treated with a combination of NAA and coconut water. There was synergistic effect of these two substances in terms of enhanced growth of shoot and fibrous root formation, Ki enhanced the growth of shoot and root. Although BA also enhanced shoot growth, root elongation was inhibited, Callus formation from shoot tip was induced by Sequential culture in media with a combination of BA and NAA, followed by modified SH medium, Three types of calluses were found: friable, water-soaked and surface calluses. All types, however, failed to show sustained growth and differentiation. The increase in shoot number sometimes occurred in cytokinin treatments. Combination of Ki and 2,4-D or modified SH and MS medium induced bud formation, but at a low frequency. Half shoot tips could be used successfully for the in vitro multiplication of &- Eeginee. Results showed that embryo and tissue culture could be more promising than the other conventional methods of multiplying S. reginae. Embryo culture could induce faster growth and higher germination rate. The limited success in callus induction and by proliferation also indicate the possibility of developing @ better method of clonal propagation and mass production of this kind of plant in the future.
541			_cSubmitted to the University Library _d01/07/1994 _eT-1492
650		0	_aCells _911592
690			_91418 _aDoctor of Philosophy _xHorticulture
700			_aValmeyor, Helen L. _942186 _eadviser
856			_uhttp://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=5a41ce75413cbbf715c4a46f8d3f90d6 _yClick here to view thesis abstract and table of contents
942			_2ddc _cMAN
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