000 | 02533nam a2200289 a 4500 | ||
---|---|---|---|
003 | OSt | ||
005 | 20240807095049.0 | ||
008 | 070525s19uu xx 00 eng d | ||
040 | _cCvSU Main Campus Library | ||
041 | 0 | _aeng | |
082 | 0 | 4 |
_a631.53 _bSi1 1980 |
100 | 1 | 0 |
_aSienes, Alicia G. _eauthor |
245 | 1 | 0 |
_aClonal propagation of Phalaenopsis equestris (Schauver) Reichb. F. and some selected Phalaenopsis hybrids using floral buds and floral stalk bud cuttings in vitro / _cAlicia G. Sienes |
260 | 0 |
_aLos Ba�os, Laguna, _c1980. _bCavite State University- Main Campus, |
|
300 |
_a124p. : ill. ; 28 cm. _billustrations ; _c cm. |
||
336 |
_2rdacontent _atext |
||
337 |
_2rdamedia _aunmediated |
||
338 |
_2rdacarrier _avolume |
||
500 | _aThesis (M.S. - - Horticulture) University of the Philippines, College, Laguna. | ||
504 | _aIncludes bibliographical references. | ||
520 | 3 | _aSIENES, ALICIA G, University of the Philippines at Los Bafios, August 1980. Clonal Propagation of Phalaenopsis equestris (Schauver) Reichb. £. and Some Selected Phalaenopsis Hybrids Using Floral and Floral Stalk Bud Cuttings in Vitro. Major Professor: Dr. Helen L. Valmayor The study was undertaken to (1) investigate the most expedient means of cloning Phalaenopsis equestris and some Phalaenopsis hybrids in vitro; (2) determine which basic media and growth regulator supplements would best Support the growth of Phalaenopsis explants; (3) develop a technique for vegetative propagation of Phalaenopsis Species and hybrids: and (4) become familiar with the finer points of orchid tissue culture. An inflorescence tip from Phalaenopsis equestris Produced 12 to 15 plantlets after 4 to 5 months when cultured in Vacin and Went medium Supplemented with 5 ppm NAA, 5 ppm Ki and 15% CW. Maintenance of the cultures in darkness and modification of the excision technique minimized phenolic oxidation in the cultures. Similarly, excision of apical bud meristem activated basal bud initials which continued to develop into plantlets (ca. 10 to 12 plantlets/ meristem) after 4 to 5 months culture in the same medium as those used for the inflorescence tip. | |
541 |
_cSubmitted to the University Library _d10/26/1993 _eT-1575 |
||
650 | _aPlant propagation. | ||
690 |
_91421 _aMaster of Science _xHorticulture |
||
856 |
_p80 _yClick here to view thesis abstract and table of contents _uhttp://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=c862357e08f2fd2f3b6ab964dae2c1d5 |
||
942 |
_2ddc _cMAN |
||
999 |
_c2400 _d2400 |