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008 070525s19uu xx 00 eng d
040 _cCvSU Main Campus Library
041 0 _aeng
082 0 4 _a631.53
_bSi1 1980
100 1 0 _aSienes, Alicia G.
_eauthor
245 1 0 _aClonal propagation of Phalaenopsis equestris (Schauver) Reichb. F. and some selected Phalaenopsis hybrids using floral buds and floral stalk bud cuttings in vitro /
_cAlicia G. Sienes
260 0 _aLos Ba�os, Laguna,
_c1980.
_bCavite State University- Main Campus,
300 _a124p. : ill. ; 28 cm.
_billustrations ;
_c cm.
336 _2rdacontent
_atext
337 _2rdamedia
_aunmediated
338 _2rdacarrier
_avolume
500 _aThesis (M.S. - - Horticulture) University of the Philippines, College, Laguna.
504 _aIncludes bibliographical references.
520 3 _aSIENES, ALICIA G, University of the Philippines at Los Bafios, August 1980. Clonal Propagation of Phalaenopsis equestris (Schauver) Reichb. £. and Some Selected Phalaenopsis Hybrids Using Floral and Floral Stalk Bud Cuttings in Vitro. Major Professor: Dr. Helen L. Valmayor The study was undertaken to (1) investigate the most expedient means of cloning Phalaenopsis equestris and some Phalaenopsis hybrids in vitro; (2) determine which basic media and growth regulator supplements would best Support the growth of Phalaenopsis explants; (3) develop a technique for vegetative propagation of Phalaenopsis Species and hybrids: and (4) become familiar with the finer points of orchid tissue culture. An inflorescence tip from Phalaenopsis equestris Produced 12 to 15 plantlets after 4 to 5 months when cultured in Vacin and Went medium Supplemented with 5 ppm NAA, 5 ppm Ki and 15% CW. Maintenance of the cultures in darkness and modification of the excision technique minimized phenolic oxidation in the cultures. Similarly, excision of apical bud meristem activated basal bud initials which continued to develop into plantlets (ca. 10 to 12 plantlets/ meristem) after 4 to 5 months culture in the same medium as those used for the inflorescence tip.
541 _cSubmitted to the University Library
_d10/26/1993
_eT-1575
650 _aPlant propagation.
690 _91421
_aMaster of Science
_xHorticulture
856 _p80
_yClick here to view thesis abstract and table of contents
_uhttp://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=c862357e08f2fd2f3b6ab964dae2c1d5
942 _2ddc
_cMAN
999 _c2400
_d2400