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040 _cCvSU Main Campus Library
041 0 _aeng
082 0 4 _a399.0113
_bB46 2018
100 _94626
_aBerchie, Emmanuel A.
_eadviser
245 1 0 _aHemagglutinating activity of birch flower (Broussonetia luzonical) lectin in abo blood group /
_cby Emmanuel A. Berchie, Keithlyn P. Gutierrez, and Abegail P. Rabanzo.
260 _aIndang, Cavite :
_bCavite State University- Main Campus,
_c2018.
300 _axvi, 87 pages :
_billustrations ;
_c28 cm.
336 _2rdacontent
_atext
337 _2rdamedia
_aunmediated
338 _2rdacarrier
_avolume
500 _aThesis (Bachelor of Science in Medical Technology) Cavite State University
504 _aIncludes bibliographical references.
508 _aCollege of Nursing (CON), Department of Medical Technology
520 3 _aBERCHIE, EMMANUEL A., GUTIERREZ, KEITHLYN P., RABANZO, ABEGAIL P. Hemagglutinating Activity of Birch flower (Broussonetia luzonica) Lectin in ABO Blood Group. Research Study. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. May 2018, Adviser: Hosea L. Matel, PhD. and Annie M. Ramos, RMT, RN, MD. The extensive application and variety of uses of lectins express the necessity to isolate lectins from indigenous sources since lectins are very expensive. One of the possible native sources of lectin is the birch flower (Broussonetia luzonica) which is commonly known as "himbabao". The study was conducted to determine the presence or absence of hemagglutination in birch flower extracts using microtiter plate (MTP) — based hemagglutination; determine the strength of agglutination of ABO blood antigen through microtiter plate (MTP) — based hemagglutination; characterize the hemagglutinating activity of lectin obtained from birch flower in terms of human blood type specificity and effect of storage time on agglutination activity; and determine significant difference between the microtiter plate (MTP) — based hemagglutination of extracts of O. 15M NaC1 in IX PBS and phosphate buffer saline (PBS) solution. In this research, the lectin was extracted using 0.15M sodium chloride (NaCI) in lX phosphate buffered saline (PBS) solution and PBS solution alone. The isolation and purification of lectin was done by ammonium sulfate precipitation at 40% to 80% saturation. Microliter plate (MTP) P) based hemagglutination using human ABO erythrocytes was performed in order to determine the presence of lectin. Consequently, the birch flower lectin was categorized as a non-blood type specific lectin since it agglutinated all the human blood types A, B, 0 and AB. The 80(') ammonium sulfate precipitate accompanied by 0.15M NaCl in IX 1)13S gave highest titer value among the other extracts with 2048 in type 13 and AB, followed by type A with 1024. The stability of lectin activity was determined after using, protein fractions with one-week, one-month and two-month shelf life. The peak of the lectin activity shown in the study was observed after one month storage of the protein fractions and gradual loss of activity was noted after second month of storage under refrigeration.
541 _cSubmitted to the University Library
_dMay 27, 2019
_eRS-849
650 0 _94627
_aBlood
_xAgglutination
650 0 _94628
_aLectins
690 _91856
_aBachelor of Science in Medical Technology
700 _94630
_aGutierrez, Keithlyn P.
_eauthor
700 _94631
_aRabanzo, Abegail P.
_eauthor
700 _91609
_aMatel, Hosea dL.
_eadviser
700 _92555
_aRamos, Annie M.
_eadviser
856 _p80
_yClick here to view abstract and table of contents
_uhttp://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=70f7e0fb23c6d1a9e26b650d223a9189
942 _2ddc
_cMAN
999 _c26411
_d26411