000			02900nam a2200325 4500
003			OSt
005			20230411094402.0
008			211017b |||||||| |||| 00| 0 eng d
040			_cCvSU Main Campus Library
041	0		_aeng
082	0	4	_a595.4 _bSa4 2019
100			_912441 _aSamaniego, Cherrymay O. _eauthor
245	1	0	_aMolecular detection of tick-borne flaviviruses in blood of Cattle in Cavite, Philippines / _cby Cherrymay O. Samaniego.
260			_aIndang, Cavite : _bCavite State University- Main Campus, _c2019.
300			_axiv, 47 pages : _billustrations ; _c28 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
500			_aThesis (Doctor of Veterinary Medicine) Cavite State University.
504			_aIncludes bibliographical references.
508			_aCollege of Veterinary Medicine and Biological Science (CVMBS), Department of Clinical and Population Health
520	3		_aSAMANIEGO, CHERRYMAY O. MOLECULAR DETECTION OF TICK- BORNE FLAVIVIRUSES IN BLOOD OF CATTLE IN CAVITE, PHILIPPINES. Thesis, Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. 2019. Adviser: Melbourne R. Talactac, DVM, MS, PhD. This study was conducted to detect the presence of tick- borne Flaviviruses (TBFV) in cattle from selected farms in Cavite. A total of one hundred blood samples were collected from the dorsal ear vein of cattle from different municipalities in Cavite. RNA samples extracted from the blood were used for complementary DNA (cDNA) synthesis. The cDNAs were initially used for the detection of mammalian Actin gene and later on, for the NS5 gene coding region conserved in TBFV using conventional Polymerase Chain Reaction (PCR). All blood samples were positive for mammalian Actin gene, however, TBFV were not detected. The result of this study suggested that TBFV may not be present in the areas where the samples were collected. Occurence of previous infection is possible but as a limitation of the study, PCR only detects present viremia in an animal through amplification of the target gene. The author recommends that further studies be conducted with larger sample size. Moreover, the time of collection should cover the warmer season because the presence of ticks in the area is important in spreading the virus. Lastly, animals should have tick infestation to be included in the study and serological tests should be utilized for improved detection.
541			_cSubmitted to the University Library _dJune 24, 2019 _eT-8113
650		0	_93593 _aTicks
650		0	_93533 _aCattle
690			_966 _aDoctor of Veterinary Medicine
700			_912442 _aTalactac, Melbourne R. _eadviser
856			_uhttp://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=ab13704adda39bc84470154bb86296c2 _yClick here to view thesis abstract and table of contents
942			_2ddc _cMAN
999			_c34633 _d34633