Fungi associated with black pepper (Piper nigrum, Linn.) cacao (Theobroma cacao, Linn.) and cucumber (Cucumis sativus, Linn.) seeds : their isolation and identification /

Villa, Divina R. de

Fungi associated with black pepper (Piper nigrum, Linn.) cacao (Theobroma cacao, Linn.) and cucumber (Cucumis sativus, Linn.) seeds : their isolation and identification / by Divina R. de Villa. - Indang, Cavite, Cavite State University- Main Campus, 1991. - xii, 46p. : ill. ; 28 cm. illustrations ; cm.

Thesis (BSA - - Crop Protection) Don Severino Agricultural College.

Includes bibliographical references.

DE VILLA, DIVINA RELACION, "Fungi Associated with Black Pepper (Piper Rigrum, Linn.), Cacao (Theobroma Cacao Linn.) and Cucumber (Cucumis sativus, Linn.) Seeds: Their Isolation and Identification". B.S. Thesis, BSA, Crop Protection, Don Severino Agricultural College, April 1991, Adviser: Mrs. Angelina S. Ordonez.
Fungi associated with the seeds of cacao (Theobroma cacao, Linn.), cucumber (Cucumis sativus, Linn.) and black pepper (Piper nigrum, Linn.) were identified using agar and blotter tests. Both methods were found to be equally efficient for the detection of fungi. Three petri plates from both tests were used and seeded at the rate of 10 and 25 seeds per plate, respectively. Cacao seeds which had been stored for 379 days and had a moisture content of 7% were found infected with Rhizopus, Penicillium and Lasiodi lodia using agar and blotter tests. Rhizopus and Fusarium were commonly found on black pepper seeds which had been stored for 440 days and with a moisture content of 25% and 9% for agar and blotter tests, respectively. Cucumber seeds stored for 310 days were infected with Rhizopus, Fusarium, Geotrichum and Colletotrichum. The cucumber seeds used in agar and blotter tests had a moisture content of 38% and 24%, respectively.
The most predominant genera of fungi invaders were Rhizopus and Penicillium for cacao seeds and Rhino us and Fusarium for black pepper and cucumber seeds. Rhizopus was consistently present on the seeds of cacao, cucumber and black pepper.



Black Pepper (Plant)
Fungi
Cacao
Cucumber

633.84 / V71 1991
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