Determination of the adsorptive property of eggshell charcoal poder against Escherichia coli for water purification /

Presto, Allan Christopher

Determination of the adsorptive property of eggshell charcoal poder against Escherichia coli for water purification / by Allan Christopher Presto, Florisa Anne Nueva Luviano and Lyka Luviano. - Indang, Cavite : Cavite State University- Main Campus, 2018. - xiii, 82 pages : illustrations ; 28 cm.

Research Study (Bachelor of Science in Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON) College of Nursing (CON)

LUVIANO, LYKA, NUEVA, FLORISA ANNE P., PRESTO, ALLAN CHRISTOPHER N., DETERMINATION OF THE ADSORPTIVE PROPERTY OF EGGSHELL CHARCOAL POWDER AGAINST Escherichia coli FOR WATER PURIFICATION. Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, November 2018. Adviser: John Jeron B. Datoy, RMT

The study was conducted at the Department of Medical Technology College of Nursing Cavite State University - Indang Campus from June to September 2018.

It aimed to: 1) determine the potency of the ECP for the purification of water; 2)determine the efficacy of the ECP against E. coli; 3) determine the time it takes for the ECP to reach its optimal adsorptive capacity; and 4) determine if ECP can be an
alternative to activated charcoal.

Eggshells were gathered from the researchers’ household and activated charcoal was bought from Trece Martires City, Cavite. The activated charcoal was used as the positive control. Eggshell charcoal powder was produced by dry heating at 270 degrees Celsius for two hours, direct heat was applied. Suspension of £. coli was prepared, as well as the different treatments needed in this study. 0.5 McFarland was used as a standard.

The optimal adsorptive capacity was then observed by performing a Standard Plate Count method with three replications by inoculating a sample from each treatment to an EMB agar every 15 minutes for one hour.

The research design used was Quasi-experimental. The gathered data were recorded; interpreted and statistically analyzed using weighted mean and two-way
ANOVA.

The results of the study indicate that in terms of optimal adsorptive capacity, Eggshell Charcoal Powder (ECP) is 3.42x10* CFU/mL of E. coli at 30 minutes while Activated Charcoal (AC) only adsorbed 2.50 x10” CFU/mL at 45 minutes. ECP was more potent than AC. Treatment 1 (5g) of ECP adsorbed more bacteria (2.02x10° CFU/mL) than Treatment 1 (5g) of AC (9.30x10” CFU/mL). ECP was more efficient and takes less time to obtain its optimal adsorptive capacity than AC. Therefore, ECP can be an alternative to AC.




Water purification

628.162 / P92 2018
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