Molecular detection of Anaplasma marginale in cattle from selected farm in Cavite using ploymerase chain reaction /
Tapawan, Lennox Pinili
Molecular detection of Anaplasma marginale in cattle from selected farm in Cavite using ploymerase chain reaction / Lennox Pinili Tapawan. - Indang, Cavite : Cavite State University- Main Campus, 2019. - xiii , 50 pages : illustrations ; 28 cm.
Thesis (Doctor of Veterinary Medicine) Cavite State University and Technology.
Includes bibliographical references.
College of Veterinary Medicine and Biomedical Sciences College of Veterinary Medicine and Biomedical Sciences
TAPAWAN, LENNOX P. Molecular Detection of Anaplasma marginale in Cattle from Selected Farms in Cavite Using Polymerase Chain Reaction. Undergraduate Thesis, Doctor of Veterinary Medicine, Cavite State Unversity, Indang, Cavite. May 2018. Adviser: Emmanuel R. Mago DVM, MS
The study was conducted to detect the presence of Anaplasma marginate in cattle from selected farms in Cavite. One hundred blood samples of cattle were collected from different municipalities in Cavite. The template DNA was extracted using the PREP DNA/RNA kit (Life Science, Germany). Mammalian actin gene served as internal control of the study. DNA samples positive for Anaplasma app. was further screened for A. marginate using A. marginate specific primers. The detection rate was correlated to different risk factors such as sex, age, backyard or commercial, type of feeding operation, breed, topography, and acaricidal treatment. It was found out that there is a 66 % detection rate of Anaplasma marginate in Cavite. Based on the results of the study, there is a significant association between three topographical areas in Cavite with the lowland areas having the highest detection rate (88%). However, no association between different age groups, sex, type of production, type of feeding management and used of acaricide were observed. The results of the study show that there is high incidence of Anaplasma marginate infections in Cavite. The study also suggests that A. marginate infections in cattle is endemic in Cavite.
Cattle
636.2 / T16 2018
Molecular detection of Anaplasma marginale in cattle from selected farm in Cavite using ploymerase chain reaction / Lennox Pinili Tapawan. - Indang, Cavite : Cavite State University- Main Campus, 2019. - xiii , 50 pages : illustrations ; 28 cm.
Thesis (Doctor of Veterinary Medicine) Cavite State University and Technology.
Includes bibliographical references.
College of Veterinary Medicine and Biomedical Sciences College of Veterinary Medicine and Biomedical Sciences
TAPAWAN, LENNOX P. Molecular Detection of Anaplasma marginale in Cattle from Selected Farms in Cavite Using Polymerase Chain Reaction. Undergraduate Thesis, Doctor of Veterinary Medicine, Cavite State Unversity, Indang, Cavite. May 2018. Adviser: Emmanuel R. Mago DVM, MS
The study was conducted to detect the presence of Anaplasma marginate in cattle from selected farms in Cavite. One hundred blood samples of cattle were collected from different municipalities in Cavite. The template DNA was extracted using the PREP DNA/RNA kit (Life Science, Germany). Mammalian actin gene served as internal control of the study. DNA samples positive for Anaplasma app. was further screened for A. marginate using A. marginate specific primers. The detection rate was correlated to different risk factors such as sex, age, backyard or commercial, type of feeding operation, breed, topography, and acaricidal treatment. It was found out that there is a 66 % detection rate of Anaplasma marginate in Cavite. Based on the results of the study, there is a significant association between three topographical areas in Cavite with the lowland areas having the highest detection rate (88%). However, no association between different age groups, sex, type of production, type of feeding management and used of acaricide were observed. The results of the study show that there is high incidence of Anaplasma marginate infections in Cavite. The study also suggests that A. marginate infections in cattle is endemic in Cavite.
Cattle
636.2 / T16 2018