Determination of insecticidal activity from sulasi (Ocicum sanctum Linn.) leaves /

Hernandez, Kaara Katishe R.

Determination of insecticidal activity from sulasi (Ocicum sanctum Linn.) leaves / Kaara Katishe R. Hernandez and Riobeth M. Panganiban. - Indang, Cavite : Cavite State University- Main Campus, 2004. - xiv, 57 pages : illustrations ; 28 cm.

Thesis (Bachelor of Science in Chemistry) Cavite State University.

Includes bibliographical references.

College of Arts and Science (CAS) College of Arts and Science (CAS)

HERNANDEZ, KAARA KATISHE R. and PANGANIBAN, RIOBETH M., “Determination of Insecticidal Activity from Sulasi (Ocimum Sanctum Linn.) Leaves”, an undergraduate thesis of the Bachelor of Science in Chemistry, , Cavite State University, Indang, Cavite. Adviser: Mrs. Ammie P. Ferrer.

This study was conducted to determine the insecticidal activity of sulasi crude extract against A. egypti. Specifically, it aimed to perform extraction of crude extract from sulasi leaves, determine the effectiveness against A. egypti through bioassay, compare the effectiveness of crude extract to bayothrin (baygon), a commercial insecticides, determine the physical properties of the active crude extract and perform the phytochemical test of active crude extract of sulasi leaves.

Two kilograms of coarsely ground sulasi leaves were collected from Bailen, Cavite on July, 2003 and were extracted through sequential extraction from different solvent of increasing polarity: petroleum ether, dichloromethane, 1:1 (v/v) chloroform- methanol, methanol and water. The crude extract yield of petroleum ether, dichloromethane, chloroform-methanol, methanol and water was calculated to be 2.0165%, 2.1375%, 1.916%, 2.0125% and 2.761% respectively.

The crude extract has the properties of dark green, smooth texture and menthol scent. It is insoluble in water but soluble in all organic solvents (carbon tetrachloride, acetone, methanol, dichloromethane, chloroform, ethanol and hexane) at room temperature. This indicates that the sulasi crude extract was non-polar due to its solubility characteristics.

Phytochemical screening showed that the sulasi crude extract contained organic compounds such as alkaloids, tannins and flavonoids; whereas, insecticidal analysis showed that the sulasi crude extract exhibited insecticidal activity against A .aegypti.



Plants, Medicinal
Sulasi

581.61 / H43 2004
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