Molecular detection of severe fever with thrombocytopenia virus and thogoto virus in blood of cattle from selected farms in Cavite using polymerase chain reaction /
Fenomeno, Carlo Erwin R.
Molecular detection of severe fever with thrombocytopenia virus and thogoto virus in blood of cattle from selected farms in Cavite using polymerase chain reaction / by Carlo Erwin R. Fenomeno. - Indang, Cavite : Cavite State University- Main Campus, 2019. - xiii, 42 pages : illustrations ; 28 cm
Thesis (Doctor of Veterinary Medicine) Cavite State University.
Includes bibliographical references.
College of Veterinary Medicine and Biomedical Sciences (CVMBS) College of Veterinary Medicine and Biomedical Sciences (CVMBS)
FENOMENO, CARLO ERWIN R. Molecular Detection of Severe Fever with Thrombocytopenia Virus and Thogoto Virus in Blood of Cattle from Selected Farms in Cavite using Polymerase Chain Reaction. Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang Cavite. May 2019. Adviser: Dr. Melbourne R. Talactac.
This study is a preliminary molecular-based detection of SFTSV and THOV from blood of cattle in the province of Cavite. Blood samples were collected from lowland, central hilly and highland areas of Cavite. Collection of samples was done by collecting blood from the ear veins of cattle. Out of the 100 blood samples obtained for the study, thirty-four samples (34) were collected from low land, forty-three (43) from central hilly and twenty-three (23) from high land area, respectively. Detection of Actin gene using PCR showed 100% detection rate for all the samples.
However, SFTSV and THOV were not detected in all samples using PCR. In conclusion, this preliminary study suggests that SFTSV and THOV were not present during the collection periods in the selected cattle farms in Cavite. The absence of these viruses in the province is most likely due to the absence of competent vectors and limitation of the method used in this study.
Based on the results of the study, it is recommended to conduct the sample collection in the months of October to March where migratory birds migrate due to winter season or alternatively during summer season when ticks are active. It is also recommended to conduct the sample collection in islands, forest, and provinces in the periphery of the Philippines, where migratory birds stay and may transmit the virus. Lastly, the use of serological tests such as enzyme-linked immunosorbent assay (ELISA) for the detection of exposed animals is highly recommended since it can detect specific antibodies from previous infections.
Molecular microbiology
Thrombocytopenia virus
Thogoto virus
Virus diseases--Molecular diagnosis
Animal viruses
579.2 / F36 2019
Molecular detection of severe fever with thrombocytopenia virus and thogoto virus in blood of cattle from selected farms in Cavite using polymerase chain reaction / by Carlo Erwin R. Fenomeno. - Indang, Cavite : Cavite State University- Main Campus, 2019. - xiii, 42 pages : illustrations ; 28 cm
Thesis (Doctor of Veterinary Medicine) Cavite State University.
Includes bibliographical references.
College of Veterinary Medicine and Biomedical Sciences (CVMBS) College of Veterinary Medicine and Biomedical Sciences (CVMBS)
FENOMENO, CARLO ERWIN R. Molecular Detection of Severe Fever with Thrombocytopenia Virus and Thogoto Virus in Blood of Cattle from Selected Farms in Cavite using Polymerase Chain Reaction. Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang Cavite. May 2019. Adviser: Dr. Melbourne R. Talactac.
This study is a preliminary molecular-based detection of SFTSV and THOV from blood of cattle in the province of Cavite. Blood samples were collected from lowland, central hilly and highland areas of Cavite. Collection of samples was done by collecting blood from the ear veins of cattle. Out of the 100 blood samples obtained for the study, thirty-four samples (34) were collected from low land, forty-three (43) from central hilly and twenty-three (23) from high land area, respectively. Detection of Actin gene using PCR showed 100% detection rate for all the samples.
However, SFTSV and THOV were not detected in all samples using PCR. In conclusion, this preliminary study suggests that SFTSV and THOV were not present during the collection periods in the selected cattle farms in Cavite. The absence of these viruses in the province is most likely due to the absence of competent vectors and limitation of the method used in this study.
Based on the results of the study, it is recommended to conduct the sample collection in the months of October to March where migratory birds migrate due to winter season or alternatively during summer season when ticks are active. It is also recommended to conduct the sample collection in islands, forest, and provinces in the periphery of the Philippines, where migratory birds stay and may transmit the virus. Lastly, the use of serological tests such as enzyme-linked immunosorbent assay (ELISA) for the detection of exposed animals is highly recommended since it can detect specific antibodies from previous infections.
Molecular microbiology
Thrombocytopenia virus
Thogoto virus
Virus diseases--Molecular diagnosis
Animal viruses
579.2 / F36 2019