Isolation, identification and biological control activity of endophytic bacteria associated with Coffee leaves /

Villanueva, Ericka Jean S.

Isolation, identification and biological control activity of endophytic bacteria associated with Coffee leaves / by Erica Jean S. Villanueva. - Indang, Cavite, Cavite State University - Main Campus 2013. - xii, 70 pages : illustrations ; 28 cm.

Thesis ( BS Biology ) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS) College of Arts and Science (CAS)

VILLANUEVA, ERICKA JEAN SACALA, March 2013, "Isolation, Identification and Biological Control Activity of Endophytic Bacteria Associated with Coffee Leaves". Undergraduate Thesis, Bachelor of Science in Biology, Major in General Biology, Cavite State University, Indang, Cavite. Adviser: Dr. Yolanda A. Ilagan
This study was done to isolate and identify bacterial endophytes present in four varieties of coffee. Specifically, this aimed to differentiate the bacterial endophytes from four varieties of coffee based on their morphological and physiological characteristics; and determine which among these bacterial endophytes can serve as effective biocontrol agents against Ralstonia solanacearum, Aspergillus niger, and Erwinia carotovora. Fifty-seven isolates were obtained from coffee leaves collected in Amadeo (n = 20), Alfonso (n = 17), and Indang (n = 20). Based on morphological, cultural and physiological tests, the isolates were identified as Enterobacter aerogenes and Enterobacter cloacae, Serratia marsecens, Bacillus sp, Alcaligenes sp, and Aminobacter sp. These bacteria are found in coffee regardless of town and varieties sampled. Four out of five genera exhibited an inhibitory effect against A. niger but none to R. solanacearum and E. carotovora.



Bacteriology

589.9 / V71 2013
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