MARC details
| 000 -LEADER |
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| fixed length control field |
03168nam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20220731182155.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
200623s19uu xx 00 eng d |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CvSU Main Campus Library |
| 041 0# - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
630 |
| Item number |
M81 2017 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Mojares, Maarten Czar Legaspi |
| Relator term |
author |
| 9 (RLIN) |
16031 |
| 245 10 - TITLE STATEMENT |
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| Title |
Molecular detection of Anaplasma spp. on ticks collected from cattle and pasture in Cavite using polymerase chain reaction / |
| Statement of responsibility, etc. |
by Maarten Czar Legaspi Mojares. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Indang, Cavite: |
| Name of publisher, distributor, etc. |
Cavite State University - Main Campus |
| Date of publication, distribution, etc. |
2017. |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xii, 58 pages |
| Other physical details |
illustrations ; |
| Dimensions |
28 cm. |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 500 ## - GENERAL NOTE |
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| General note |
Thesis (Doctor of Veterinary Medicine) Cavite State University. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references. |
| 508 ## - CREATION/PRODUCTION CREDITS NOTE |
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| College and Department |
College of Veterinary Medicine and Biomedical Sciences (VETMED) |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
MOJARES, MAARTEN CZAR L., PCR Detection of Mt-rrs Gene and Anaplasma spp. among Ticks Collected from Cattle and Pasture in Cavite. Undergraduate Thesis/Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite,2017. Adviser: Dr Emmanuel R. Mago <br/>This study was conducted as a preliminary survey of the presence of tick borne pathogen Anaplasma spp. in different cattle farms in Cavite, Philippines using PCR. A total of 293 adult ticks and 1 larva samples were collected in which 63 (21.42%) came from lowland and 231 (78.57%) from central hilly areas in Cavite. Ticks were identified and classified according to species, sex, development stage and topography. Eighty nine samples were tested using blackPrep Tick Kit and 205 samples using Boiling method for Mt-rrs gene detection. Eighty (89.89% ) samples used in blackPrep Tick Kit were positive for the gene while 85 (41.46°A) samples including 1 larva in Boiling method. All 165 mt-rrs gene positive samples were tested for Anaplasma spp. detection but failed to exhibit distinct band on the 513 by mark indicating that the samples were not Anaplasma spp. BlackPrep Tick DNA/RNA kit provided a much higher detection rate than using Boiling method in detection of Mt-rrs gene. The presence of three genes in Rhipicephalus microplus namely CK187220, CV437619 and TC18492 might have been a contributing factor in failure to detect Anapalsma spp. Use of Serum collected from cattle would greatly help in detecting the presence of Anaplasma spp. in farms in Cavite. Different species of ticks should be used as sample to avoid bias in the result. The use of gradient PCR would increase the specificity of detection thus providing a more reasonable result.<br/> |
| 541 ## - IMMEDIATE SOURCE OF ACQUISITION NOTE |
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| Method of acquisition |
Submitted to the University Library<br/> |
| Date of acquisition |
08/24/2017 |
| Accession number |
T-7284 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Cattletick |
| 9 (RLIN) |
16032 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Cattle |
| General subdivision |
Parasites. |
| 9 (RLIN) |
3533 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Tick-borne diseases in animals. |
| 9 (RLIN) |
16033 |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) |
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| 9 (RLIN) |
66 |
| Course or Program |
Doctor of Veterinary Medicine |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
3534 |
| Personal name |
Mago, Emmanuel R. |
| Relator term |
adviser |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Electronic File |
<a href="http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=b7a8a9ab6cf2ddc6a36f10b19878ca77">http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=b7a8a9ab6cf2ddc6a36f10b19878ca77</a> |
| Link text |
Click here to view thesis abstract and table of contents |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Theses / Manuscripts |
